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Literature summary for 1.14.19.2 extracted from

  • Schlueter, P.M.; Xu, S.; Gagliardini, V.; Whittle, E.; Shanklin, J.; Grossniklaus, U.; Schiestl, F.P.
    Stearoyl-acyl carrier protein desaturases are associated with floral isolation in sexually deceptive orchids (2011), Proc. Natl. Acad. Sci. USA, 108, 5696-5701.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.19.2

Cloned(Commentary)

Cloned (Comment) Organism
OeSAD2 is not expressed as a soluble protein Ophrys sphegodes

Organism

Organism UniProt Comment Textmining
Ophrys sphegodes
-
-
-
Ophrys sphegodes subsp. sphegodes
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Ophrys sphegodes
-
flower
-
 Ophrys sphegodes subsp. sphegodes
-
leaf
-
 Ophrys sphegodes
-
leaf
-
 Ophrys sphegodes subsp. sphegodes
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information in vitro analysis of the enzyme does not show any product Ophrys sphegodes ?
-
 ?
additional information lack of soluble OeSAD2 protein expression precludes its in vitro analysis Ophrys sphegodes ?
-
 ?
additional information OsSAD2 is active both on 18:0-[acyl-carrier protein] DELTA9 and 16:0-[acyl-carrier protein] DELTA4 substrates producing 18:1 DELTA9 and 16:1 DELTA4 products Ophrys sphegodes ?
-
 ?

Synonyms

Synonyms Comment Organism
OeSAD2
-
 Ophrys sphegodes
OeSAD3
-
 Ophrys sphegodes subsp. sphegodes
OsSAD1
-
 Ophrys sphegodes
OsSAD2
-
 Ophrys sphegodes
OsSAD3
-
 Ophrys sphegodes
stearoyl-acyl carrier protein desaturase
-
 Ophrys sphegodes
stearoyl-acyl carrier protein desaturase
-
 Ophrys sphegodes subsp. sphegodes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at Ophrys sphegodes

General Information

General Information Comment Organism
malfunction Ophrys SAD coding sequences are heterologously expressed in Arabidopsis under the control of the Cauliflower mosaic virus 35S RNA promoter. None of the transgenic plant lines complement the dwarf phenotype of homozygous ssi2 mutants. The presence of the OsSAD2 transgene is significantly associated with changes in unsaturated C18 and C16 FA levels in Arabidopsis leaf lipids, suggesting that OsSAD2 has enzymatic activity in Arabidopsis Ophrys sphegodes
malfunction Ophrys SAD coding sequences are heterologously expressed in Arabidopsis under the control of the Cauliflower mosaic virus 35S RNA promoter. OeSAD2 does not complement the dwarf phenotype of homozygous ssi2 mutants Ophrys sphegodes
malfunction Ophrys SAD coding sequences are heterologously expressed in Arabidopsis under the control of the Cauliflower mosaic virus 35S RNA promoter. OsSAD1 does not complement the dwarf phenotype of homozygous ssi2 mutants Ophrys sphegodes
physiological function SAD2 is a florally expressed barrier gene of large phenotypic effect and, possibly, a genic target of pollinator-mediated selection Ophrys sphegodes