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Literature summary for 1.14.19.22 extracted from

  • Pugh, E.L.; Kates, M.
    Characterization of a membrane-bound phospholipid desaturase system of candida lipolytica (1975), Biochim. Biophys. Acta, 380, 442-453.
    View publication on PubMed
Search for more literature for 1.14.19.22

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 at an optimal concentration of 0.1% Triton X-100, the detergent increases the rate of desaturation about three fold Yarrowia lipolytica

Inhibitors

Inhibitors Comment Organism Structure
cyanide the enzyme inhibited by cyanide but not by carbon monoxide, indicating that it required cytochrome b5 Yarrowia lipolytica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine
-
 Yarrowia lipolytica
0.25
-
 1-acyl-2-oleoyl-sn-glycero-3-phosphorylcholine pH 7.4, 37°C Yarrowia lipolytica
0.95
-
 1,2-di-oleoyl-sn-glycero-3-phosphorylcholine
-
 Yarrowia lipolytica
0.95
-
 1,2-di-oleoyl-sn-glycero-3-phosphorylcholine pH 7.4, 37°C Yarrowia lipolytica

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Yarrowia lipolytica
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-acyl-2-oleoyl-sn-glycero-3-phosphocholine + ferrocytochrome b5 + O2 + H+ Yarrowia lipolytica
-
 1-acyl-2-linoleoyl-sn-glycero-3-phosphocholine + ferricytochrome b5 + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Yarrowia lipolytica
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-di-oleoyl-sn-glycero-3-phosphorylcholine + 2 ferrocytochrome b5 + O2 + 2 H+ desaturation takes place at both position-1 and position-2. The distearoyl or dielaidoyl phosphatidylcholines are not desaturated Yarrowia lipolytica 1,2-di-linoleoyl-sn-glycero-3-phosphorylcholine + 2 ferricytochrome b5 + 2 H2O
-
 ?
1,2-di-oleoyl-sn-glycero-3-phosphorylcholine + O2 + ?
-
 Yarrowia lipolytica ?
-
 ?
1-acyl-2-oleoyl-sn-glycero-3-phosphocholine + ferrocytochrome b5 + O2 + H+
-
 Yarrowia lipolytica 1-acyl-2-linoleoyl-sn-glycero-3-phosphocholine + ferricytochrome b5 + H2O
-
 ?
1-acyl-2-oleoyl-sn-glycero-3-phosphorylcholine + 2 ferrocytochrome b5 + O2 + 2 H+
-
 Yarrowia lipolytica 1-acyl-2-linoleoyl-sn-glycero-3-phosphorylcholine + 2 ferricytochrome b5 + 2 H2O
-
 ?
additional information not: di-oleoyl phosphatidylcholine, dielaidoyl phosphatidylcholine Yarrowia lipolytica ?
-
 ?

Synonyms

Synonyms Comment Organism
oleoyl-CoA desaturase incorrect Yarrowia lipolytica

Cofactor

Cofactor Comment Organism Structure
cytochrome b5 required Yarrowia lipolytica
NAD+
-
 Yarrowia lipolytica