Application | Comment | Organism |
---|---|---|
agriculture | potential of exploiting FAD overexpression as a tool to ameliorate drought tolerance in plants | Arabidopsis thaliana |
biotechnology | potential of exploiting FAD overexpression as a tool to ameliorate drought tolerance in plants | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
gene fad8, recombinant expression in Nicotiana tabacum BY-2 cells and in transgenic tobacco plants via Agrobacterium-mediated transformation. Co-expression of Arabidopsis thaliana FAD8 and Brassica napus FAD3, EC 1.14.19.25, under the control of the 35S CaMV promoter leads to increase in 18:3, which is compensated mainly by the decrease in 18:2, but is also accompanied by a decrease in oleic acid (18:1). Additionally, there is a small but significant decrease in stearic acid (18:0) which seems to be compensated by an increase in palmitic acid (16:0). In the BY-2-FAD8 cells, the ratio of 18:3 to 18:2 is about twofold higher than that in the BY-2 cells. Fatty acid composition of leaf polar lipids of wild-type and transgenic plants, overview | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | recombinant overexpression of FAD8 increases tolerance to drought in tobacco plants and to osmotic stress in cultured cells. Ectopic overexpression of FAD8 induces an increased ratio mainly in the plastidic lipids | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | P48622 | gene FAD8 | - |
Arabidopsis thaliana Col-0 | P48622 | gene FAD8 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
root | - |
Arabidopsis thaliana | - |
Synonyms | Comment | Organism |
---|---|---|
FAD8 | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
additional information | ectopic overexpression of FAD8 induces an increased ratio mainly in the plastidic lipids. Overexpression of FAD8 leads to increased tolerance to osmotic stress (imposed by two different agents, PEG 8000 and sorbitol) and decreased tolerance to heat stress (35°C), at both cellular and whole-plant levels. FAD8-overexpressing plants can regain growth after withholding water for a duration that severely impaires the ability of wild-type plants to recover from the stress. Fatty acid composition of leaf polar lipids of wild-type and transgenic plants, overview | Arabidopsis thaliana |