Cloned (Comment) | Organism |
---|---|
gene Cf0807 or CfACET, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged ACTE in Saccharomyces cerevisiae | Cantharellus formosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the enzyme contains positionally conserved histidine box motifs that are believed to bind two active-site iron atoms and are critical to enzyme function | Cantharellus formosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Cantharellus formosus | H6Cf0807 is an acetylenase, that produces a trans DELTA12 double bond, while H6Cf0745 is a classical FAD2 desaturase. The Cantharellus formosus FAD2 homologue H6Cf0745 expression results in the production of only 16:2 DELTA9,12 and 18:2 DELTA9,12 in recombinant yeast cells supplemented with 18:2 DELTA9c,12c. Expression ofH6Cf0807 results in the production of crepenynic acid, 18:1 DELTA9c,12a. No C16 acetylenic fatty acid is detected. Supplementation with 18:2 DELTA9c,12t, alpha-linolenic acid, 18:3 DELTA9,12,15, or gamma-linolenic acid, 18:3 DELTA6,9,12, does not result in the production of other fatty acids, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cantharellus formosus | D9ZGG6 | gene Cf0807 or CfACET | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit body | - |
Cantharellus formosus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | H6Cf0807 is an acetylenase, that produces a trans DELTA12 double bond, while H6Cf0745 is a classical FAD2 desaturase. The Cantharellus formosus FAD2 homologue H6Cf0745 expression results in the production of only 16:2 DELTA9,12 and 18:2 DELTA9,12 in recombinant yeast cells supplemented with 18:2 DELTA9c,12c. Expression ofH6Cf0807 results in the production of crepenynic acid, 18:1 DELTA9c,12a. No C16 acetylenic fatty acid is detected. Supplementation with 18:2 DELTA9c,12t, alpha-linolenic acid, 18:3 DELTA9,12,15, or gamma-linolenic acid, 18:3 DELTA6,9,12, does not result in the production of other fatty acids, overview | Cantharellus formosus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ACET | - |
Cantharellus formosus |
acetylenase | - |
Cantharellus formosus |
H6Cf0807 | - |
Cantharellus formosus |
linoleoyl 12-desaturase | - |
Cantharellus formosus |
General Information | Comment | Organism |
---|---|---|
metabolism | the bifunctional desaturase and the acetylenase provide the enzymatic activities required to drive oleate through linoleate to crepenynate and the conjugated enyne (14Z)-dehydrocrepenynate, the branchpoint precursors to a major class of acetylenic natural products, biosynthesis of polyacetylenic metabolites in Basidiomycetes, overview | Cantharellus formosus |
additional information | the acetylenase gene, identified from the fungus, is phylogenetically distinct from known plant and fungal desaturases. The enzyme contains positionally conserved histidine box motifs that are believed to bind two active-site iron atoms and are critical to enzyme function | Cantharellus formosus |
physiological function | to produce the accumulated natural product dehydrocrepenynic acid, chanterelle must have both FAD2/3 desaturase, EC 1.14.19.6. and acetylenase activities, production of the trans DELTA12 double bond by H6Cf0807 | Cantharellus formosus |