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Literature summary for 1.14.19.39 extracted from

  • Chodok, P.; Eiamsa-ard, P.; Cove, D.J.; Quatrano, R.S.; Kaewsuwan, S.
    Identification and functional characterization of two DELTA12-fatty acid desaturases associated with essential linoleic acid biosynthesis in Physcomitrella patens (2013), J. Ind. Microbiol. Biotechnol., 40, 901-913.
    View publication on PubMed
Search for more literature for 1.14.19.39

Cloned(Commentary)

Cloned (Comment) Organism
gene PpFAD2-2, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, expression in Saccharomyces cerevisiae strain INVSc1 Physcomitrium patens
genes PpFAD2-1, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, expression in Saccharomyces cerevisiae strain INVSc1 Physcomitrium patens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum membrane the transmembrane protein PpFAD2-1 contains three conserved histidine-rich motifs: the HXXXH motif from amino acid residue 99 to 103 (HECGH), the HXXHH motif from amino acid residue 135 to 139 (HRRHH), and the HXXHH motif from amino acid residue 309 to 313 (HVAHH) in the C-terminus Physcomitrium patens 5789
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endoplasmic reticulum membrane the transmembrane protein PpFAD2-2 contains three conserved histidine-rich motifs: the HXXXH motif from amino acid residue 99 to 103 (HECGH), the HXXHH motif from amino acid residue 135 to 139 (HRRHH), and the HXXHH motif from amino acid residue 309 to 313 (HVAHH) in the C-terminus Physcomitrium patens 5789
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microsome
-
 Physcomitrium patens
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-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphatidylcholine + reduced cytochrome b5 + O2 Physcomitrium patens with NADH ? + cytochrome b5 + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Physcomitrium patens A9RQ27 FAD2-2; gene PpFAD2-2
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Physcomitrium patens A9RV06 FAD2-1; gene PpFAD2-1 encoding two isozymes
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidylcholine + reduced cytochrome b5 + O2 with NADH Physcomitrium patens ? + cytochrome b5 + 2 H2O
-
 ?

Synonyms

Synonyms Comment Organism
DELTA12-fatty acid desaturases
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 Physcomitrium patens
FAD2
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 Physcomitrium patens
FAD2-1
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 Physcomitrium patens
FAD2-2
-
 Physcomitrium patens
microsomal DELTA12-fatty acid desaturase
-
 Physcomitrium patens

General Information

General Information Comment Organism
evolution the microsomal DELTA12-fatty acid desaturase (FAD2) is located in the endoplasmic reticulum and uses phosphatidylcholine as acyl substrate and NADH, NADH-cytochrome b5 reductase, and cytochrome b5 as electron donors. This reaction involves concomitant reduction of molecular oxygen to water. In contrast, the plastidial DELTA12-fatty acid desaturase (FAD6) is located in the chloroplast and uses primarily glycolipids as acyl carriers and NAD(P)H, ferredoxin-NAD(P) reductase, and ferredoxin as electron donors Physcomitrium patens
additional information the isozyme contains the three histidine clusters typical of the catalytic domains of DELTA12-desaturase enzymes Physcomitrium patens