Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Bax inhibitor-1 | from Arabidopsis thaliana, BI-1 (AtBI-1) protein interacts with the enzyme. Bax inhibitor-1 is a widely conserved cell death suppressor localized in the endoplasmic reticulum membrane. AtBI-1 binds cytochrome b5-like domain-containing proteins like sphingolipid DELTA8 long-chain base desaturases SLD1 and SLD2. AtBI-1 interacts with AtSLD2 in the endoplasmic reticulum membrane. The strength of interaction with AtBI-1 is almost the same for isozymes AtSLD1 and AtSLD2. AtBI-1 is not indispensable for desaturase activity of AtSLDs | Arabidopsis thaliana | |
Bax inhibitor-1 | from Arabidopsis thaliana, BI-1 (AtBI-1) protein interacts with the enzyme. Bax inhibitor-1 is a widely conserved cell death suppressor localized in the endoplasmic reticulum membrane. AtBI-1 binds cytochrome b5-like domain-containing proteins like sphingolipid DELTA8 long-chain base desaturases SLD1 and SLD2. The AtBI-1 protein expression is induces in cold exposure. AtBI-1 contributes to synthesis of sphingolipids during cold stress by interacting with AtSLD1 in the endoplasmic reticulum membrane. The strength of interaction with AtBI-1 is almost the same for isozymes AtSLD1 and AtSLD2. AtBI-1 is not indispensable for desaturase activity of AtSLDs | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
gene AtSLD1, sequence comparisons, quantitative enzyme expression analysis, recombinant expression as cYFP fusion protein driven by cauliflower mosaic virus 35S (CaMV35S) promoter in onion epidermal cells, coexpression of AtBI-1-cYFP and AtSLD1-nYFP | Arabidopsis thaliana |
gene AtSLD2, sequence comparions, quantitative enzyme expression analysis, recombinant expression as cYFP fusion protein driven by cauliflower mosaic virus 35S (CaMV35S) promoter in onion epidermal cells | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of isozymes SLD1 and SLD2 knockout mutant, sphingolipid content in sld1sld2 and Bax inhibitor-1 atbi-1 mutant during cold treatment, overview | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum membrane | - |
Arabidopsis thaliana | 5789 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Arabidopsis thaliana | desaturation of long-chain base moieties of sphingolipid classes (ceramide, hydroxyceramide, GlcCer, and GIPC) during cold treatment, overview | ? | - |
? | |
additional information | Arabidopsis thaliana Col-0 | desaturation of long-chain base moieties of sphingolipid classes (ceramide, hydroxyceramide, GlcCer, and GIPC) during cold treatment, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q3EBF7 | - |
- |
Arabidopsis thaliana | Q9ZRP7 | - |
- |
Arabidopsis thaliana Col-0 | Q3EBF7 | - |
- |
Arabidopsis thaliana Col-0 | Q9ZRP7 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Arabidopsis thaliana | - |
leaf | - |
Arabidopsis thaliana | - |
additional information | expression of isozyme AtSLD2 is limited to flowers and siliques. AtSLD1 contributes to DELTA8 LCB desaturation in stems, flowers, leaves, siliques and roots, while isozyme AtSLD2 has a small effect on desaturation in every tissue, even in flowers and siliques, where AtSLD2 is highly expressed | Arabidopsis thaliana | - |
additional information | isozyme AtSLD1 is broadly expressed in all tissues. AtSLD1 contributes to DELTA8 LCB desaturation in stems, flowers, leaves, siliques and roots, while isozyme AtSLD2 has a small effect on desaturation in every tissue, even in flowers and siliques, where AtSLD2 is highly expressed | Arabidopsis thaliana | - |
root | - |
Arabidopsis thaliana | - |
silique | - |
Arabidopsis thaliana | - |
stem | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | desaturation of long-chain base moieties of sphingolipid classes (ceramide, hydroxyceramide, GlcCer, and GIPC) during cold treatment, overview | Arabidopsis thaliana | ? | - |
? | |
additional information | desaturation of long-chain base moieties of sphingolipid classes (ceramide, hydroxyceramide, GlcCer, and GIPC) during cold treatment, overview | Arabidopsis thaliana Col-0 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AtSLD1 | - |
Arabidopsis thaliana |
AtSLD2 | - |
Arabidopsis thaliana |
sphingolipid DELTA8 LCB desaturase | - |
Arabidopsis thaliana |
sphingolipid DELTA8 long-chain base desaturase | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome b5 | a Cb5-like domain (Cb5LD)-containing protein | Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | the expression of AtSLD2 is decreased at cold exposure | down |
Arabidopsis thaliana | the expression of AtSLD1 is increased at cold exposure | up |
General Information | Comment | Organism |
---|---|---|
physiological function | isozymes AtSLD1 and AtSLD2 desaturate the DELTA8 position of the long-chain bases (LCBs) of sphingolipids in the endoplasmic reticulum membrane, and include a cytochrome b5-like domain at the N-terminus. 90% of Arabidopsis thaliana long-chain bases contain a cis or trans double bond at the DELTA8 position, and their desaturation is catalyzed by AtSLD1 and AtSLD2 | Arabidopsis thaliana |