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Literature summary for 1.14.19.41 extracted from
- CYP710A genes encoding sterol C22-desaturase in Physcomitrella patens as molecular evidence for the evolutionary conservation of a sterol biosynthetic pathway in plants (2009), Planta, 229, 1311-1322.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in a baculovirus/Sf9 insect cell system | Physcomitrium patens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
beta-sitosterol | isoform CAP710A13, at pH 7.3 and 30°C | Physcomitrium patens |  |
| 0.0021 | - |
beta-sitosterol | isoform CAP710A14, at pH 7.3 and 30°C | Physcomitrium patens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Physcomitrium patens | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57028 | - |
x * 57028, isoform CYP710A14, calculated from amino acid sequence | Physcomitrium patens |
| 57410 | - |
x * 57410, isoform CYP710A13, calculated from amino acid sequence | Physcomitrium patens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-sitosterol + NADPH + H+ + O2 | Physcomitrium patens | - |
stigmasterol + NADP+ + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Physcomitrium patens | - |
subspecies Physcomitrella patens patens, strain Gransden Wood | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-sitosterol + NADPH + H+ + O2 | - |
Physcomitrium patens | stigmasterol + NADP+ + H2O | - |
? | |
| additional information | campesterol and 24-epi-campesterol are not accepted as substrates | Physcomitrium patens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 56000-57000, SDS-PAGE | Physcomitrium patens |
| ? | x * 57028, isoform CYP710A14, calculated from amino acid sequence | Physcomitrium patens |
| ? | x * 57410, isoform CYP710A13, calculated from amino acid sequence | Physcomitrium patens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CYP710A | - |
Physcomitrium patens |
| CYP710A13 | isoform | Physcomitrium patens |
| CYP710A14 | isoform | Physcomitrium patens |
| sterol C22-desaturase | - |
Physcomitrium patens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
beta-sitosterol | isoform CAP710A14, at pH 7.3 and 30°C | Physcomitrium patens | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Physcomitrium patens | |
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Release 2023.1 (January 2023)
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