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Literature summary for 1.14.20.1 extracted from

  • Ghag, S.K.; Brems, D.N.; Hassell, T.C.; Yeh, W.K.
    Refolding and purification of Cephalosporium acremonium deacetoxycephalosporin C synthetase/hydroxylase from granules of recombinant Escherichia coli (1996), Biotechnol. Appl. Biochem., 24 , 109-119.
    View publication on PubMed

Application

Application Comment Organism
synthesis expression in Escherichia coli, overexpression as an insoluble and inactive enzyme, elaboration of refolding scheme resulting in highliy active and moderately stable enzyme Acremonium chrysogenum

Organism

Organism UniProt Comment Textmining
Acremonium chrysogenum
-
recombinant enzyme, catalyzes both reaction of EC 1.14.20.1 and of EC 1.14.11.26
-

Purification (Commentary)

Purification (Comment) Organism
expression in Escherichia coli, overexpression as an insoluble and inactive enzyme, elaboration of refolding scheme resulting in highliy active and moderately stable enzyme Acremonium chrysogenum