Cloned (Comment) | Organism |
---|---|
all mutants are overexpressed in the baculovirus system as detergent-soluble full-length proteins | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
Y148F | mutant enzyme has cyclooxygenase activity comparable to that of the native enzyme | Homo sapiens |
Y148F/Y348F/Y385F/Y404F/Y504F | no cyclooxygenase activity detected | Homo sapiens |
Y148F/Y348F/Y404F/Y504F | mutant enzyme has specific cyclooxygenase activity approximately half that of native enzyme. Modest increase in cyclooxygenase self-inactivation rate, 2.3fold | Homo sapiens |
Y348F | mutant enzyme has specific cyclooxygenase activity approximately half that of native enzyme | Homo sapiens |
Y385F | no cyclooxygenase activity detected | Homo sapiens |
Y404F | mutant enzyme has cyclooxygenase activity comparable to that of the native enzyme. Modest increase in cyclooxygenase self-inactivation rate, 2.3fold | Homo sapiens |
Y504F | mutant enzyme has cyclooxygenase activity comparable to that of the native enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0021 | - |
arachidonate | native enzyme | Homo sapiens | |
0.0032 | - |
arachidonate | mutant enzyme Y148F | Homo sapiens | |
0.0036 | - |
arachidonate | mutant enzyme Y404F | Homo sapiens | |
0.0038 | - |
arachidonate | mutant enzyme Y348F | Homo sapiens | |
0.0042 | - |
arachidonate | mutant enzyme Y504F | Homo sapiens | |
0.0043 | - |
arachidonate | mutant enzyme Y148F/Y348F/Y404F/Y504F | Homo sapiens | |
0.02 | - |
trans-5-phenyl-4-pentenyl-1-hydroperoxide | mutant enzyme Y148F | Homo sapiens | |
0.061 | - |
trans-5-phenyl-4-pentenyl-1-hydroperoxide | mutant enzyme Y404F | Homo sapiens | |
0.072 | - |
trans-5-phenyl-4-pentenyl-1-hydroperoxide | mutant enzyme Y348F | Homo sapiens | |
0.103 | - |
trans-5-phenyl-4-pentenyl-1-hydroperoxide | mutant enzyme Y148F/Y348F/Y385F/Y404F/Y504F | Homo sapiens | |
0.138 | - |
trans-5-phenyl-4-pentenyl-1-hydroperoxide | native enzyme | Homo sapiens | |
0.339 | - |
trans-5-phenyl-4-pentenyl-1-hydroperoxide | mutant enzyme Y385F | Homo sapiens | |
0.37 | - |
trans-5-phenyl-4-pentenyl-1-hydroperoxide | mutant enzyme Y148F/Y348F/Y404F/Y504F | Homo sapiens | |
0.437 | - |
trans-5-phenyl-4-pentenyl-1-hydroperoxide | mutant enzyme Y504F | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonate + electron donor + O2 | cyclooxygenase activity | Homo sapiens | prostaglandin H2 + oxidized electron donor + H2O | - |
? | |
guaiacol + trans-5-phenyl-4-pentenyl-1-hydroperoxide | - |
Homo sapiens | ? | - |
? | |
H2O2 + N,N,N',N'-tetramethyl-p-phenylenediamine | peroxidase activity | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PGHS | - |
Homo sapiens |
prostaglandin H synthase | - |
Homo sapiens |