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Literature summary for 1.14.99.1 extracted from

  • Rogge, C.E.; Liu, W.; Wu, G.; Wang, L.H.; Kulmacz, R.J.; Tsai, A.L.
    Identification of Tyr504 as an alternative tyrosyl radical site in human prostaglandin H synthase-2 (2004), Biochemistry, 43, 1560-1568.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
all mutants are overexpressed in the baculovirus system as detergent-soluble full-length proteins Homo sapiens

Protein Variants

Protein Variants Comment Organism
Y148F mutant enzyme has cyclooxygenase activity comparable to that of the native enzyme Homo sapiens
Y148F/Y348F/Y385F/Y404F/Y504F no cyclooxygenase activity detected Homo sapiens
Y148F/Y348F/Y404F/Y504F mutant enzyme has specific cyclooxygenase activity approximately half that of native enzyme. Modest increase in cyclooxygenase self-inactivation rate, 2.3fold Homo sapiens
Y348F mutant enzyme has specific cyclooxygenase activity approximately half that of native enzyme Homo sapiens
Y385F no cyclooxygenase activity detected Homo sapiens
Y404F mutant enzyme has cyclooxygenase activity comparable to that of the native enzyme. Modest increase in cyclooxygenase self-inactivation rate, 2.3fold Homo sapiens
Y504F mutant enzyme has cyclooxygenase activity comparable to that of the native enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0021
-
arachidonate native enzyme Homo sapiens
0.0032
-
arachidonate mutant enzyme Y148F Homo sapiens
0.0036
-
arachidonate mutant enzyme Y404F Homo sapiens
0.0038
-
arachidonate mutant enzyme Y348F Homo sapiens
0.0042
-
arachidonate mutant enzyme Y504F Homo sapiens
0.0043
-
arachidonate mutant enzyme Y148F/Y348F/Y404F/Y504F Homo sapiens
0.02
-
trans-5-phenyl-4-pentenyl-1-hydroperoxide mutant enzyme Y148F Homo sapiens
0.061
-
trans-5-phenyl-4-pentenyl-1-hydroperoxide mutant enzyme Y404F Homo sapiens
0.072
-
trans-5-phenyl-4-pentenyl-1-hydroperoxide mutant enzyme Y348F Homo sapiens
0.103
-
trans-5-phenyl-4-pentenyl-1-hydroperoxide mutant enzyme Y148F/Y348F/Y385F/Y404F/Y504F Homo sapiens
0.138
-
trans-5-phenyl-4-pentenyl-1-hydroperoxide native enzyme Homo sapiens
0.339
-
trans-5-phenyl-4-pentenyl-1-hydroperoxide mutant enzyme Y385F Homo sapiens
0.37
-
trans-5-phenyl-4-pentenyl-1-hydroperoxide mutant enzyme Y148F/Y348F/Y404F/Y504F Homo sapiens
0.437
-
trans-5-phenyl-4-pentenyl-1-hydroperoxide mutant enzyme Y504F Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidonate + electron donor + O2 cyclooxygenase activity Homo sapiens prostaglandin H2 + oxidized electron donor + H2O
-
?
guaiacol + trans-5-phenyl-4-pentenyl-1-hydroperoxide
-
Homo sapiens ?
-
?
H2O2 + N,N,N',N'-tetramethyl-p-phenylenediamine peroxidase activity Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
PGHS
-
Homo sapiens
prostaglandin H synthase
-
Homo sapiens