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Literature summary for 1.14.99.14 extracted from

  • Petric, S.; Hakki, T.; Bernhardt, R.; Zigon, D.; Cresnar, B.
    Discovery of a steroid 11alpha-hydroxylase from Rhizopus oryzae and its biotechnological application (2010), J. Biotechnol., 150, 428-437.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene CYP509C12, cloning from a cDNA library, DNA and amino acid sequence determination and analysis, phylogenetic analysis, and recombinant expression in Pichia pastoris, coexpression with NAD(P)H-dependent reductase also from Rhizopus oryzae, which improves electron transfer to CYP509C12 and thus increases the production of 11-hydroxyprogesterone by 7fold. The engineered strain displays total bioconversion of progesterone into 11alpha-hydroxyprogesterone and small amounts of 6beta-hydroxyprogesterone Rhizopus arrhizus

Protein Variants

Protein Variants Comment Organism
additional information gene CYP509C12, production of a recombinant Pichia pastoris engieered to coexpress gene CYP509C12, ecoding the enzyme with the NAD(P)H-dependent reductase also from Rhizopus oryzae, to improve electron transfer to CYP509C12 and thus increases the production of 11-hydroxyprogesterone by 7fold. The engineered strain displays total bioconversion of progesterone into 11alpha-hydroxyprogesterone and small amounts of 6beta-hydroxyprogesterone and is useful for biotechnologic applications Rhizopus arrhizus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
11-deoxycorticosterone + AH2 + O2 Rhizopus arrhizus
-
? + A + H2O
-
?
11-deoxycorticosterone + AH2 + O2 Rhizopus arrhizus RA 99-880
-
? + A + H2O
-
?
11-deoxycortisol + AH2 + O2 Rhizopus arrhizus
-
? + A + H2O
-
?
11-deoxycortisol + AH2 + O2 Rhizopus arrhizus RA 99-880
-
? + A + H2O
-
?
progesterone + AH2 + O2 Rhizopus arrhizus
-
11alpha-hydroxyprogesterone + A + H2O
-
?
progesterone + AH2 + O2 Rhizopus arrhizus RA 99-880
-
11alpha-hydroxyprogesterone + A + H2O
-
?
testosterone + AH2 + O2 Rhizopus arrhizus
-
11alpha-hydroxytestosterone + A + H2O
-
?
testosterone + AH2 + O2 Rhizopus arrhizus RA 99-880
-
11alpha-hydroxytestosterone + A + H2O
-
?

Organism

Organism UniProt Comment Textmining
Rhizopus arrhizus
-
gene CYP509C12
-
Rhizopus arrhizus RA 99-880
-
gene CYP509C12
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
11-deoxycorticosterone + AH2 + O2
-
Rhizopus arrhizus ? + A + H2O
-
?
11-deoxycorticosterone + AH2 + O2
-
Rhizopus arrhizus RA 99-880 ? + A + H2O
-
?
11-deoxycortisol + AH2 + O2
-
Rhizopus arrhizus ? + A + H2O
-
?
11-deoxycortisol + AH2 + O2
-
Rhizopus arrhizus RA 99-880 ? + A + H2O
-
?
additional information hydroxylations take place predominantly at 11alpha and 6beta positions of the steroid ring system Rhizopus arrhizus ?
-
?
additional information hydroxylations take place predominantly at 11alpha and 6beta positions of the steroid ring system Rhizopus arrhizus RA 99-880 ?
-
?
progesterone + AH2 + O2
-
Rhizopus arrhizus 11alpha-hydroxyprogesterone + A + H2O
-
?
progesterone + AH2 + O2
-
Rhizopus arrhizus RA 99-880 11alpha-hydroxyprogesterone + A + H2O
-
?
testosterone + AH2 + O2
-
Rhizopus arrhizus 11alpha-hydroxytestosterone + A + H2O
-
?
testosterone + AH2 + O2
-
Rhizopus arrhizus RA 99-880 11alpha-hydroxytestosterone + A + H2O
-
?

Expression

Organism Comment Expression
Rhizopus arrhizus CYP509C12 expression is induced by progesterone up