Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.99.22 extracted from

  • Johnson, P.; Rees, H.H
    The mechanism of C-20 hydroxylation of alpha-ecdysone in the desert locust, Schistocerca gregaria (1977), Biochem. J., 168, 513-520.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
CO partially reversible by irradiation with monochromatic light Schistocerca gregaria
KCN 25% inhibition Schistocerca gregaria
Metyrapone 2-methyl-1,2-di-3'-pyridylpropan-1-one, up to 80% inhibition Schistocerca gregaria

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00125
-
alpha-ecdysone
-
Schistocerca gregaria

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion membrane bound Schistocerca gregaria 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ecdysone + NADPH + O2 Schistocerca gregaria serves as precursor of ecdysterone, the active moulting hormone in larval insects 20-hydroxyecdysone + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Schistocerca gregaria
-
desert locust
-

Reaction

Reaction Comment Organism Reaction ID
ecdysone + reduced acceptor + O2 = 20-hydroxyecdysone + acceptor + H2O NADPH-dependent monooxygenase containing cytochrome P-450, a heme-thiolate protein Schistocerca gregaria

Source Tissue

Source Tissue Comment Organism Textmining
fat body larval Schistocerca gregaria
-
malpighian tubule
-
Schistocerca gregaria
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Schistocerca gregaria
0.00000562
-
malphigian tubules, 10000 x g pellet Schistocerca gregaria

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-ecdysone + NADPH + O2
-
Schistocerca gregaria 20-hydroxyecdysone + NADP+ + H2O
-
?
ecdysone + NADPH + O2 serves as precursor of ecdysterone, the active moulting hormone in larval insects Schistocerca gregaria 20-hydroxyecdysone + NADP+ + H2O
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Schistocerca gregaria

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Schistocerca gregaria

Cofactor

Cofactor Comment Organism Structure
cytochrome P450 essential Schistocerca gregaria
NADPH
-
Schistocerca gregaria