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Literature summary for 1.14.99.22 extracted from

  • Feyereisen, R.; Durst, F.
    Ecdysterone biosynthesis: a microsomal cytochrome-P-450-linked ecdysone 20-monooxygenase from tissues of the African migratory locust (1978), Eur. J. Biochem., 88, 37-47.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
20-hydroxyecdysone competitive inhibition Locusta migratoria migratorioides
7,8-Benzoflavone
-
Locusta migratoria migratorioides
CO partially reversible by irradiation with monochromatic light Locusta migratoria migratorioides
cytochrome c
-
Locusta migratoria migratorioides
ecdysterone competitive inhibition Locusta migratoria migratorioides
Metyrapone
-
Locusta migratoria migratorioides
NADP+ competitive inhibition Locusta migratoria migratorioides
NEM
-
Locusta migratoria migratorioides
nicotinamide
-
Locusta migratoria migratorioides
oxidized cytochrome c reversed by KCN Locusta migratoria migratorioides
PCMB
-
Locusta migratoria migratorioides
Phospholipase A
-
Locusta migratoria migratorioides
Phospholipase C
-
Locusta migratoria migratorioides
Piperonylbutoxide
-
Locusta migratoria migratorioides
SKF 525A
-
Locusta migratoria migratorioides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00027
-
ecdysone
-
Locusta migratoria migratorioides
0.0105
-
NADPH
-
Locusta migratoria migratorioides

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome bound to sedimentable membrane Locusta migratoria migratorioides
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ecdysone + NADPH + O2 Locusta migratoria migratorioides serves as precursor of ecdysterone, the active moulting hormone in larval insects 20-hydroxyecdysone + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Locusta migratoria migratorioides
-
L. migratoria migratorioides: african migration locust
-

Reaction

Reaction Comment Organism Reaction ID
ecdysone + reduced acceptor + O2 = 20-hydroxyecdysone + acceptor + H2O NADPH-dependent monooxygenase containing cytochrome P-450, a heme-thiolate protein Locusta migratoria migratorioides

Source Tissue

Source Tissue Comment Organism Textmining
fat body larval Locusta migratoria migratorioides
-
malpighian tubule
-
Locusta migratoria migratorioides
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00000017
-
fat body Locusta migratoria migratorioides
0.00000041
-
mid- and hindgut Locusta migratoria migratorioides
0.00000075
-
microsomes, fat body Locusta migratoria migratorioides
0.00000115
-
malphigian tubules Locusta migratoria migratorioides
0.000003
-
microsomes, malphigian tubules Locusta migratoria migratorioides

Storage Stability

Storage Stability Organism
-30°C, microsomal preparations with 30% v/v glycerol Locusta migratoria migratorioides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-ecdysone + NADPH + O2 3beta,14alpha,22,25-pentahydroxy-5beta-cholest-7-en-6-one, the 5alpha-epimer of ecdysone, 2beta-acetoxyecdysone, 3-hydroxyecdysone, 3-dehydro-ecdysone or ecdysterone are not hydroxylated Locusta migratoria migratorioides 20-hydroxyecdysone + NADP+ + H2O beta-ecdysone, ecdysterone ?
ecdysone + NADPH + O2 serves as precursor of ecdysterone, the active moulting hormone in larval insects Locusta migratoria migratorioides 20-hydroxyecdysone + NADP+ + H2O
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Locusta migratoria migratorioides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6
-
-
Locusta migratoria migratorioides

Cofactor

Cofactor Comment Organism Structure
cytochrome P450 essential Locusta migratoria migratorioides
NADH increase of activity, synergism together with NADPH, but cannot substitute NADPH as cofactor Locusta migratoria migratorioides
NADPH ultimate hydrogen donor, cannot be substituted by NADH or ascorbate Locusta migratoria migratorioides
NADPH NADP+ is not effective together with either malate, isocitrate, succinate, but with glucose-6-phosphate 25% of the activity is restored Locusta migratoria migratorioides