Inhibitors | Comment | Organism | Structure |
---|---|---|---|
20-hydroxyecdysone | competitive inhibition | Locusta migratoria migratorioides | |
7,8-Benzoflavone | - |
Locusta migratoria migratorioides | |
CO | partially reversible by irradiation with monochromatic light | Locusta migratoria migratorioides | |
cytochrome c | - |
Locusta migratoria migratorioides | |
ecdysterone | competitive inhibition | Locusta migratoria migratorioides | |
Metyrapone | - |
Locusta migratoria migratorioides | |
NADP+ | competitive inhibition | Locusta migratoria migratorioides | |
NEM | - |
Locusta migratoria migratorioides | |
nicotinamide | - |
Locusta migratoria migratorioides | |
oxidized cytochrome c | reversed by KCN | Locusta migratoria migratorioides | |
PCMB | - |
Locusta migratoria migratorioides | |
Phospholipase A | - |
Locusta migratoria migratorioides | |
Phospholipase C | - |
Locusta migratoria migratorioides | |
Piperonylbutoxide | - |
Locusta migratoria migratorioides | |
SKF 525A | - |
Locusta migratoria migratorioides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00027 | - |
ecdysone | - |
Locusta migratoria migratorioides | |
0.0105 | - |
NADPH | - |
Locusta migratoria migratorioides |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | bound to sedimentable membrane | Locusta migratoria migratorioides | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ecdysone + NADPH + O2 | Locusta migratoria migratorioides | serves as precursor of ecdysterone, the active moulting hormone in larval insects | 20-hydroxyecdysone + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Locusta migratoria migratorioides | - |
L. migratoria migratorioides: african migration locust | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ecdysone + reduced acceptor + O2 = 20-hydroxyecdysone + acceptor + H2O | NADPH-dependent monooxygenase containing cytochrome P-450, a heme-thiolate protein | Locusta migratoria migratorioides |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fat body | larval | Locusta migratoria migratorioides | - |
malpighian tubule | - |
Locusta migratoria migratorioides | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00000017 | - |
fat body | Locusta migratoria migratorioides |
0.00000041 | - |
mid- and hindgut | Locusta migratoria migratorioides |
0.00000075 | - |
microsomes, fat body | Locusta migratoria migratorioides |
0.00000115 | - |
malphigian tubules | Locusta migratoria migratorioides |
0.000003 | - |
microsomes, malphigian tubules | Locusta migratoria migratorioides |
Storage Stability | Organism |
---|---|
-30°C, microsomal preparations with 30% v/v glycerol | Locusta migratoria migratorioides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-ecdysone + NADPH + O2 | 3beta,14alpha,22,25-pentahydroxy-5beta-cholest-7-en-6-one, the 5alpha-epimer of ecdysone, 2beta-acetoxyecdysone, 3-hydroxyecdysone, 3-dehydro-ecdysone or ecdysterone are not hydroxylated | Locusta migratoria migratorioides | 20-hydroxyecdysone + NADP+ + H2O | beta-ecdysone, ecdysterone | ? | |
ecdysone + NADPH + O2 | serves as precursor of ecdysterone, the active moulting hormone in larval insects | Locusta migratoria migratorioides | 20-hydroxyecdysone + NADP+ + H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Locusta migratoria migratorioides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | - |
- |
Locusta migratoria migratorioides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | essential | Locusta migratoria migratorioides | |
NADH | increase of activity, synergism together with NADPH, but cannot substitute NADPH as cofactor | Locusta migratoria migratorioides | |
NADPH | ultimate hydrogen donor, cannot be substituted by NADH or ascorbate | Locusta migratoria migratorioides | |
NADPH | NADP+ is not effective together with either malate, isocitrate, succinate, but with glucose-6-phosphate 25% of the activity is restored | Locusta migratoria migratorioides |