Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | increase of activity | Schistocerca gregaria |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
20-hydroxyecdysone | competitive inhibition | Schistocerca gregaria | |
Ca2+ | - |
Schistocerca gregaria | |
CO | partially reversible by irradiation with monochromatic light | Schistocerca gregaria | |
EDTA | - |
Schistocerca gregaria | |
Metyrapone | - |
Schistocerca gregaria | |
Mg2+ | - |
Schistocerca gregaria | |
Non-ionic detergents | Tween 20, Tween 80 and Triton X-100 | Schistocerca gregaria | |
Tris/HCl-buffer | - |
Schistocerca gregaria |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00071 | - |
ecdysone | - |
Schistocerca gregaria |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | membrane bound | Schistocerca gregaria | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ecdysone + NADPH + O2 | Schistocerca gregaria | serves as precursor of ecdysterone, the active moulting hormone in larval insects | 20-hydroxyecdysone + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schistocerca gregaria | - |
desert locust | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ecdysone + reduced acceptor + O2 = 20-hydroxyecdysone + acceptor + H2O | NADPH-dependent monooxygenase containing cytochrome P-450, a heme-thiolate protein | Schistocerca gregaria |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
malpighian tubule | - |
Schistocerca gregaria | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0000183 | - |
- |
Schistocerca gregaria |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-ecdysone + NADPH + O2 | - |
Schistocerca gregaria | 20-hydroxyecdysone + NADP+ + H2O | - |
? | |
ecdysone + NADPH + O2 | serves as precursor of ecdysterone, the active moulting hormone in larval insects | Schistocerca gregaria | 20-hydroxyecdysone + NADP+ + H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Schistocerca gregaria |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | 8 | potassium phosphate buffer | Schistocerca gregaria |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | essential | Schistocerca gregaria | |
NADH | increase of activity, synergism together with NADPH, but cannot substitute NADPH as cofactor | Schistocerca gregaria | |
NADPH | - |
Schistocerca gregaria |