Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.99.22 extracted from

  • Weirich, G.F.
    Ecdysone 20-monooxygenase (1985), Methods Enzymol., 3, 454-458.
No PubMed abstract available

General Stability

General Stability Organism
bovine serum albumin stabilizes Manduca sexta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00183
-
ecdysone mitochondrial enzyme system Manduca sexta

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ecdysone + NADPH + O2 Manduca sexta serves as precursor of ecdysterone, the active moulting hormone in larval insects 20-hydroxyecdysone + NADP+ + H2O
-
?
ecdysone + NADPH + O2 Manduca sexta alpha-ecdysone, a secretory product of prothoracic glands 20-hydroxyecdysone + NADP+ + H2O
-
?
ecdysone + NADPH + O2 Manduca sexta last step in biosynthesis of insect molting hormone 20-hydroxyecdysone + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Manduca sexta
-
tobacco hornworm
-

Purification (Commentary)

Purification (Comment) Organism
-
Manduca sexta

Reaction

Reaction Comment Organism Reaction ID
ecdysone + reduced acceptor + O2 = 20-hydroxyecdysone + acceptor + H2O NADPH-dependent monooxygenase containing cytochrome P-450, a heme-thiolate protein Manduca sexta

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0000466
-
mitochondrial enzyme system Manduca sexta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-ecdysone + NADPH + O2
-
Manduca sexta 20-hydroxyecdysone + NADP+ + H2O
-
?
ecdysone + NADPH + O2 serves as precursor of ecdysterone, the active moulting hormone in larval insects Manduca sexta 20-hydroxyecdysone + NADP+ + H2O
-
?
ecdysone + NADPH + O2 alpha-ecdysone, a secretory product of prothoracic glands Manduca sexta 20-hydroxyecdysone + NADP+ + H2O
-
?
ecdysone + NADPH + O2 last step in biosynthesis of insect molting hormone Manduca sexta 20-hydroxyecdysone + NADP+ + H2O
-
?

Cofactor

Cofactor Comment Organism Structure
cytochrome P450 essential Manduca sexta
NADPH
-
Manduca sexta