General Stability | Organism |
---|---|
instable, linear activity under standard assay conditions for 10 min | Spinacia oleracea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CO | partially reversible by irradiation with monochromatic light | Spinacia oleracea | |
additional information | in pure N2 atmosphere, enzyme activity is 50% inhibited due to missing oxygen | Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
ecdysone | - |
Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Spinacia oleracea | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ecdysone + NADPH + O2 | Spinacia oleracea | - |
20-hydroxyecdysone + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
not: purification of the enzyme from microsomes failed due to unstability of the enzyme | Spinacia oleracea |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ecdysone + reduced acceptor + O2 = 20-hydroxyecdysone + acceptor + H2O | NADPH-dependent monooxygenase containing cytochrome P-450, a heme-thiolate protein | Spinacia oleracea | |
ecdysone + reduced acceptor + O2 = 20-hydroxyecdysone + acceptor + H2O | ecdysteroid biosynthesis regulation | Spinacia oleracea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Spinacia oleracea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0021 | - |
microsomes | Spinacia oleracea |
Storage Stability | Organism |
---|---|
-80°C, quick frozen membranes, 15% glycerol v/v, at least 1 month | Spinacia oleracea |
4°C to -20°C storage for prolonged periods not possible due to unstability of the enzyme, appreciable loss of activity | Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-ecdysone + NADPH + O2 | - |
Spinacia oleracea | 20-hydroxyecdysone + NADP+ + H2O | - |
? | |
ecdysone + NADPH + O2 | - |
Spinacia oleracea | 20-hydroxyecdysone + NADP+ + H2O | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | essential | Spinacia oleracea | |
NADPH | ultimate hydrogen donor, cannot be substituted by NADH or ascorbate | Spinacia oleracea |