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Literature summary for 1.14.99.22 extracted from

  • Grebenok, R.J.; Galbraith, D.W.; Benveniste, I.; Feyereisen, R.
    Ecdysone 20-monooxygenase , a cytochrome P-450 enzyme from spinach, Spinacia oleracea (1996), Phytochemistry, 42, 927-933.
No PubMed abstract available

General Stability

General Stability Organism
instable, linear activity under standard assay conditions for 10 min Spinacia oleracea

Inhibitors

Inhibitors Comment Organism Structure
CO partially reversible by irradiation with monochromatic light Spinacia oleracea
additional information in pure N2 atmosphere, enzyme activity is 50% inhibited due to missing oxygen Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
ecdysone
-
Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Spinacia oleracea
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ecdysone + NADPH + O2 Spinacia oleracea
-
20-hydroxyecdysone + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
not: purification of the enzyme from microsomes failed due to unstability of the enzyme Spinacia oleracea

Reaction

Reaction Comment Organism Reaction ID
ecdysone + reduced acceptor + O2 = 20-hydroxyecdysone + acceptor + H2O NADPH-dependent monooxygenase containing cytochrome P-450, a heme-thiolate protein Spinacia oleracea
ecdysone + reduced acceptor + O2 = 20-hydroxyecdysone + acceptor + H2O ecdysteroid biosynthesis regulation Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Spinacia oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0021
-
microsomes Spinacia oleracea

Storage Stability

Storage Stability Organism
-80°C, quick frozen membranes, 15% glycerol v/v, at least 1 month Spinacia oleracea
4°C to -20°C storage for prolonged periods not possible due to unstability of the enzyme, appreciable loss of activity Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-ecdysone + NADPH + O2
-
Spinacia oleracea 20-hydroxyecdysone + NADP+ + H2O
-
?
ecdysone + NADPH + O2
-
Spinacia oleracea 20-hydroxyecdysone + NADP+ + H2O
-
?

Cofactor

Cofactor Comment Organism Structure
cytochrome P450 essential Spinacia oleracea
NADPH ultimate hydrogen donor, cannot be substituted by NADH or ascorbate Spinacia oleracea