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Literature summary for 1.14.99.22 extracted from

  • Weirich, G.F.; Williams, V.P.; Feldlaufer, M.F.
    Ecdysone 20-hydroxylation in Manduca sexta midgut: kinetic parameters of mitochondrial and microsomal ecdysone 20-monooxygenases (1996), Arch. Insect Biochem. Physiol., 31, 305-312.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000000367
-
ecdysone microsomal enzyme Manduca sexta
0.0000163
-
ecdysone mitochondrial enzyme Manduca sexta

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Manduca sexta
-
-
mitochondrion
-
Manduca sexta 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ecdysone + NADPH + O2 Manduca sexta serves as precursor of ecdysterone, the active moulting hormone in larval insects 20-hydroxyecdysone + NADP+ + H2O
-
?
ecdysone + NADPH + O2 Manduca sexta last step in biosynthesis of insect molting hormone 20-hydroxyecdysone + NADP+ + H2O
-
?
ecdysone + NADPH + O2 Manduca sexta midgut microsomes show higher specific activity with 2fold higher Vmax, while total activity is 8-10fold higher in mitochondria 20-hydroxyecdysone + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Manduca sexta
-
tobacco hornworm
-

Reaction

Reaction Comment Organism Reaction ID
ecdysone + reduced acceptor + O2 = 20-hydroxyecdysone + acceptor + H2O microsomal enzyme system contains NADPH-dependent cytochrome P-450 reductase without a second electron carrier type Manduca sexta
ecdysone + reduced acceptor + O2 = 20-hydroxyecdysone + acceptor + H2O a cytochrome P-450-dependent steroid hydroxylase as part of the mitochondrial enzyme system with both ferredoxin and cytochrome P-450 components Manduca sexta

Source Tissue

Source Tissue Comment Organism Textmining
fat body enzyme is located in mitochondrion Manduca sexta
-
integument
-
Manduca sexta
-
malpighian tubule
-
Manduca sexta
-
midgut larval, enzyme is located in mitochondrion and microsome Manduca sexta
-
salivary gland
-
Manduca sexta
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-ecdysone + NADPH + O2
-
Manduca sexta 20-hydroxyecdysone + NADP+ + H2O
-
?
ecdysone + NADPH + O2 serves as precursor of ecdysterone, the active moulting hormone in larval insects Manduca sexta 20-hydroxyecdysone + NADP+ + H2O
-
?
ecdysone + NADPH + O2 last step in biosynthesis of insect molting hormone Manduca sexta 20-hydroxyecdysone + NADP+ + H2O
-
?
ecdysone + NADPH + O2 midgut microsomes show higher specific activity with 2fold higher Vmax, while total activity is 8-10fold higher in mitochondria Manduca sexta 20-hydroxyecdysone + NADP+ + H2O
-
?

Cofactor

Cofactor Comment Organism Structure
cytochrome P450 essential Manduca sexta
Ferredoxin isozyme in mitochondria Manduca sexta
NADPH
-
Manduca sexta