Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diquat dibromide | - |
Parasarcophaga argyrostoma | |
diquat dibromide | - |
Sarcophaga bullata | |
Tween 80 | microsomal isozyme | Parasarcophaga argyrostoma | |
Tween 80 | microsomal isozyme | Sarcophaga bullata |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | microsomal isozyme | Sarcophaga bullata | - |
- |
microsome | microsomal isozyme | Parasarcophaga argyrostoma | - |
- |
mitochondrion | mitochondrial isozyme | Sarcophaga bullata | 5739 | - |
mitochondrion | mitochondrial isozyme | Parasarcophaga argyrostoma | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ecdysone + NADPH + O2 | Sarcophaga bullata | - |
20-hydroxyecdysone + NADP+ + H2O | - |
? | |
ecdysone + NADPH + O2 | Parasarcophaga argyrostoma | - |
20-hydroxyecdysone + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Parasarcophaga argyrostoma | - |
flesh-fly | - |
Sarcophaga bullata | - |
flesh-fly | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ecdysone + reduced acceptor + O2 = 20-hydroxyecdysone + acceptor + H2O | 2 different isozymes of mitochondria and microsomes may have different inducers, electron donors, endogenous inhibitors, and physiological optima | Sarcophaga bullata | |
ecdysone + reduced acceptor + O2 = 20-hydroxyecdysone + acceptor + H2O | 2 different isozymes of mitochondria and microsomes may have different inducers, electron donors, endogenous inhibitors, and physiological optima | Parasarcophaga argyrostoma |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
body wall | - |
Sarcophaga bullata | - |
body wall | - |
Parasarcophaga argyrostoma | - |
fat body | third instar wandering stage larvae 67-68% of total activity, after white prepuparial stage activity is inactivated in fat body | Sarcophaga bullata | - |
fat body | third instar wandering stage larvae 67-68% of total activity, after white prepuparial stage activity is inactivated in fat body | Parasarcophaga argyrostoma | - |
malpighian tubule | - |
Sarcophaga bullata | - |
malpighian tubule | - |
Parasarcophaga argyrostoma | - |
ring gland | - |
Sarcophaga bullata | - |
ring gland | - |
Parasarcophaga argyrostoma | - |
trachea | 8-9% of total activity | Sarcophaga bullata | - |
trachea | 8-9% of total activity | Parasarcophaga argyrostoma | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00000000617 | - |
fat body | Parasarcophaga argyrostoma |
0.0000000103 | - |
fat body | Sarcophaga bullata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-ecdysone + NADPH + O2 | - |
Sarcophaga bullata | 20-hydroxyecdysone + NADP+ + H2O | - |
? | |
alpha-ecdysone + NADPH + O2 | - |
Parasarcophaga argyrostoma | 20-hydroxyecdysone + NADP+ + H2O | - |
? | |
ecdysone + NADPH + O2 | - |
Sarcophaga bullata | 20-hydroxyecdysone + NADP+ + H2O | - |
? | |
ecdysone + NADPH + O2 | - |
Parasarcophaga argyrostoma | 20-hydroxyecdysone + NADP+ + H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
microsomal isozyme | Sarcophaga bullata |
30 | - |
microsomal isozyme | Parasarcophaga argyrostoma |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
microsomal isozyme | Sarcophaga bullata |
7.2 | - |
microsomal isozyme | Parasarcophaga argyrostoma |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | essential | Sarcophaga bullata | |
cytochrome P450 | essential | Parasarcophaga argyrostoma | |
Ferredoxin | isozyme in mitochondria | Sarcophaga bullata | |
Ferredoxin | isozyme in mitochondria | Parasarcophaga argyrostoma | |
NADPH | dependent on | Sarcophaga bullata | |
NADPH | dependent on | Parasarcophaga argyrostoma |