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Literature summary for 1.14.99.22 extracted from

  • Darvas, B.; Rees, H.H.; Hoggard, N.
    Ecdysone 20-monooxygenase systems in flesh-flies (diptera: sarcophagidae), Neobellieria bullata and Parasarcophaga argyrostoma (1993), Comp. Biochem. Physiol. B, 105, 765-773.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
diquat dibromide
-
Parasarcophaga argyrostoma
diquat dibromide
-
Sarcophaga bullata
Tween 80 microsomal isozyme Parasarcophaga argyrostoma
Tween 80 microsomal isozyme Sarcophaga bullata

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome microsomal isozyme Sarcophaga bullata
-
-
microsome microsomal isozyme Parasarcophaga argyrostoma
-
-
mitochondrion mitochondrial isozyme Sarcophaga bullata 5739
-
mitochondrion mitochondrial isozyme Parasarcophaga argyrostoma 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ecdysone + NADPH + O2 Sarcophaga bullata
-
20-hydroxyecdysone + NADP+ + H2O
-
?
ecdysone + NADPH + O2 Parasarcophaga argyrostoma
-
20-hydroxyecdysone + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Parasarcophaga argyrostoma
-
flesh-fly
-
Sarcophaga bullata
-
flesh-fly
-

Reaction

Reaction Comment Organism Reaction ID
ecdysone + reduced acceptor + O2 = 20-hydroxyecdysone + acceptor + H2O 2 different isozymes of mitochondria and microsomes may have different inducers, electron donors, endogenous inhibitors, and physiological optima Sarcophaga bullata
ecdysone + reduced acceptor + O2 = 20-hydroxyecdysone + acceptor + H2O 2 different isozymes of mitochondria and microsomes may have different inducers, electron donors, endogenous inhibitors, and physiological optima Parasarcophaga argyrostoma

Source Tissue

Source Tissue Comment Organism Textmining
body wall
-
Sarcophaga bullata
-
body wall
-
Parasarcophaga argyrostoma
-
fat body third instar wandering stage larvae 67-68% of total activity, after white prepuparial stage activity is inactivated in fat body Sarcophaga bullata
-
fat body third instar wandering stage larvae 67-68% of total activity, after white prepuparial stage activity is inactivated in fat body Parasarcophaga argyrostoma
-
malpighian tubule
-
Sarcophaga bullata
-
malpighian tubule
-
Parasarcophaga argyrostoma
-
ring gland
-
Sarcophaga bullata
-
ring gland
-
Parasarcophaga argyrostoma
-
trachea 8-9% of total activity Sarcophaga bullata
-
trachea 8-9% of total activity Parasarcophaga argyrostoma
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00000000617
-
fat body Parasarcophaga argyrostoma
0.0000000103
-
fat body Sarcophaga bullata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-ecdysone + NADPH + O2
-
Sarcophaga bullata 20-hydroxyecdysone + NADP+ + H2O
-
?
alpha-ecdysone + NADPH + O2
-
Parasarcophaga argyrostoma 20-hydroxyecdysone + NADP+ + H2O
-
?
ecdysone + NADPH + O2
-
Sarcophaga bullata 20-hydroxyecdysone + NADP+ + H2O
-
?
ecdysone + NADPH + O2
-
Parasarcophaga argyrostoma 20-hydroxyecdysone + NADP+ + H2O
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
microsomal isozyme Sarcophaga bullata
30
-
microsomal isozyme Parasarcophaga argyrostoma

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
microsomal isozyme Sarcophaga bullata
7.2
-
microsomal isozyme Parasarcophaga argyrostoma

Cofactor

Cofactor Comment Organism Structure
cytochrome P450 essential Sarcophaga bullata
cytochrome P450 essential Parasarcophaga argyrostoma
Ferredoxin isozyme in mitochondria Sarcophaga bullata
Ferredoxin isozyme in mitochondria Parasarcophaga argyrostoma
NADPH dependent on Sarcophaga bullata
NADPH dependent on Parasarcophaga argyrostoma