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Literature summary for 1.14.99.22 extracted from

  • Winter, J.; Eckerskorn, C.; Waditschatka, R.; Kayser, H.
    A microsomal ecdysone-binding cytochrome P-450 from the insect Locusta migratoria purified by sequential use of type-II and type-I ligands (2001), Biol. Chem., 382, 1541-1549.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloned CYP6H1 cDNA is probably identical with purified enzyme Locusta migratoria migratorioides

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Locusta migratoria migratorioides
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
? * 60000, SDS-PAGE Locusta migratoria migratorioides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ecdysone + NADPH + O2 Locusta migratoria migratorioides
-
20-hydroxyecdysone + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Locusta migratoria migratorioides
-
migration locust
-

Purification (Commentary)

Purification (Comment) Organism
solubilization of the enzyme from microsomes with detergent Synperonic NP10 Locusta migratoria migratorioides

Source Tissue

Source Tissue Comment Organism Textmining
malpighian tubule last instar larvae Locusta migratoria migratorioides
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-ecdysone + NADPH + O2
-
Locusta migratoria migratorioides 20-hydroxyecdysone + NADP+ + H2O
-
?
ecdysone + NADPH + O2
-
Locusta migratoria migratorioides 20-hydroxyecdysone + NADP+ + H2O
-
?

Subunits

Subunits Comment Organism
? ? * 60000, SDS-PAGE Locusta migratoria migratorioides

Cofactor

Cofactor Comment Organism Structure
cytochrome P450 essential Locusta migratoria migratorioides
NADPH
-
Locusta migratoria migratorioides