Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.99.22 extracted from

  • Mitchell, M.J.; Brescia, A.I.; Smith, S.L.; Morgan, E.D.
    Effects of the compounds 2-methoxynaphthoquinone, 2-propoxynaphthoquinone, and 2-isopropoxynaphthoquinone on ecdysone 20-monooxygenase activity (2007), Arch. Insect Biochem. Physiol., 66, 45-52.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-isopropoxy-1,4-naphthoquinone synthetic compound Aedes aegypti
2-isopropoxy-1,4-naphthoquinone synthetic compound Drosophila melanogaster
2-isopropoxy-1,4-naphthoquinone synthetic compound Manduca sexta
2-methoxy-1,4-naphthoquinone isolated from the leaves of Impatiens glandulifera Aedes aegypti
2-methoxy-1,4-naphthoquinone isolated from the leaves of Impatiens glandulifera Drosophila melanogaster
2-methoxy-1,4-naphthoquinone isolated from the leaves of Impatiens glandulifera Manduca sexta
2-propoxy-1,4-naphthoquinone synthetic compound Aedes aegypti
2-propoxy-1,4-naphthoquinone synthetic compound Drosophila melanogaster
2-propoxy-1,4-naphthoquinone synthetic compound Manduca sexta
additional information inhibition profiles Aedes aegypti
additional information inhibition profiles Drosophila melanogaster
additional information inhibition profiles Manduca sexta

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ a cytochrome P450-dependent hydroxylase Drosophila melanogaster
Fe2+ a cytochrome P450-dependent hydroxylase Aedes aegypti
Fe2+ a cytochrome P450-dependent hydroxylase Manduca sexta

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ecdysone + AH2 + O2 Drosophila melanogaster the enzyme is responsible for the conversion of the molting hormone ecdysone to its more physiologically active metabolite, 20-hydroxyecdysone 20-hydroxyecdysone + A + H2O
-
?
ecdysone + AH2 + O2 Aedes aegypti the enzyme is responsible for the conversion of the molting hormone ecdysone to its more physiologically active metabolite, 20-hydroxyecdysone 20-hydroxyecdysone + A + H2O
-
?
ecdysone + AH2 + O2 Manduca sexta the enzyme is responsible for the conversion of the molting hormone ecdysone to its more physiologically active metabolite, 20-hydroxyecdysone 20-hydroxyecdysone + A + H2O
-
?

Organism

Organism UniProt Comment Textmining
Aedes aegypti
-
females
-
Drosophila melanogaster
-
-
-
Manduca sexta
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
abdomen
-
Aedes aegypti
-
fat body from fifth instar larvae Manduca sexta
-
larva fifth instar larvae Manduca sexta
-
larva wandering stage third instar larvae Drosophila melanogaster
-
midgut from fifth instar larvae Manduca sexta
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Drosophila melanogaster
additional information
-
-
Aedes aegypti
additional information
-
-
Manduca sexta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ecdysone + AH2 + O2 the enzyme is responsible for the conversion of the molting hormone ecdysone to its more physiologically active metabolite, 20-hydroxyecdysone Drosophila melanogaster 20-hydroxyecdysone + A + H2O
-
?
ecdysone + AH2 + O2 the enzyme is responsible for the conversion of the molting hormone ecdysone to its more physiologically active metabolite, 20-hydroxyecdysone Aedes aegypti 20-hydroxyecdysone + A + H2O
-
?
ecdysone + AH2 + O2 the enzyme is responsible for the conversion of the molting hormone ecdysone to its more physiologically active metabolite, 20-hydroxyecdysone Manduca sexta 20-hydroxyecdysone + A + H2O
-
?
ecdysone + NADPH + H+ + O2
-
Drosophila melanogaster 20-hydroxyecdysone + NADP+ + H2O
-
?
ecdysone + NADPH + H+ + O2
-
Aedes aegypti 20-hydroxyecdysone + NADP+ + H2O
-
?
ecdysone + NADPH + H+ + O2
-
Manduca sexta 20-hydroxyecdysone + NADP+ + H2O
-
?

Synonyms

Synonyms Comment Organism
E-20-M
-
Drosophila melanogaster
E-20-M
-
Aedes aegypti
E-20-M
-
Manduca sexta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Drosophila melanogaster
7.5
-
assay at Aedes aegypti
7.5
-
assay at Manduca sexta

Cofactor

Cofactor Comment Organism Structure
cytochrome P450 a cytochrome P450-dependent hydroxylase Drosophila melanogaster
cytochrome P450 a cytochrome P450-dependent hydroxylase Aedes aegypti
cytochrome P450 a cytochrome P450-dependent hydroxylase Manduca sexta
NADPH
-
Drosophila melanogaster
NADPH
-
Aedes aegypti