Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Manduca sexta | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ecdysone + NADPH + H+ + O2 | Manduca sexta | - |
20-hydroxyecdysone + NADP+ + H2O | - |
? | |
additional information | Manduca sexta | E20-M preferentially uses NADPH in catalyzing ecdysone conversion to the physiologically active molting hormone, 20-hydroxyecdysone | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Manduca sexta | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fat body | - |
Manduca sexta | - |
midgut | during the ten-day developmental period preceding the larval-pupal molt, fifth larval instar, onset of wandering behavior and a 50fold increase in ecdysone 20-monooxygenase activity occur | Manduca sexta | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ecdysone + NADPH + H+ + O2 | - |
Manduca sexta | 20-hydroxyecdysone + NADP+ + H2O | - |
? | |
additional information | E20-M preferentially uses NADPH in catalyzing ecdysone conversion to the physiologically active molting hormone, 20-hydroxyecdysone | Manduca sexta | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
E20-M | - |
Manduca sexta |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | preferred cofactor | Manduca sexta |
General Information | Comment | Organism |
---|---|---|
additional information | physiological and developmental significance of the mitochondrial, NADPH-forming energy-linked transhydrogenations, that catalyzes a reversible hydride ion transfer between NADP(H) and NAD(H), for E20-M activity. NADPH-forming activity occurs as a nonenergy- or energy-linked activity with energy for the latter derived from either electron transport-dependent NADH or succinate utilization, or ATP hydrolysis by Mg2+-dependent ATPase, overview | Manduca sexta |
physiological function | essential need for conversion of the molting hormone, ecdysone, to its physiologically active form, 20-hydroxyecdysone by E20-M during the post-embryonic development | Manduca sexta |