General Stability | Organism |
---|---|
45 mM dithiothreitol affords partial protection against denaturation | Brevibacillus brevis |
50% glycerol affords partial protection against denaturation | Brevibacillus brevis |
dialysis of extracts in cold, 4°C, against phosphate buffer, pH 7.0, 0.05 M, for 4 h, 90% activity loss | Brevibacillus brevis |
enzyme is completely blocked by anaerobic conditions | Brevibacillus brevis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
FMN | 85% inhibition at 1.7 mM, 2 min preincubation | Brevibacillus brevis | |
Na2As2O3 | partial inhibition | Brevibacillus brevis | |
NaF | 53% inhibition at 1.7 mM, 5 min preincubation | Brevibacillus brevis | |
NaN3 | 53% inhibition at 1.7 mM, 5 min preincubation | Brevibacillus brevis | |
NiCl2 | 47% inhibition at 1.7 mM | Brevibacillus brevis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Brevibacillus brevis | 5737 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxypyridine + NADH + O2 | Brevibacillus brevis | - |
2,5-dihydroxypyridine + NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevibacillus brevis | - |
isolated by enrichment on isonicotinic acid | - |
Storage Stability | Organism |
---|---|
-10°C or 0°C, overnight, complete loss of activity | Brevibacillus brevis |
-20°C, Tris HCl buffer, pH 7.4, 0.05 M, 10% ethanol, 50% glycerol, 0.045 M dithiothreitol, 1 mM 2-mercaptoethanol, stable for 16 d | Brevibacillus brevis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxypyridine + NADH + O2 | - |
Brevibacillus brevis | 2,5-dihydroxypyridine + NAD+ + H2O | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.2 | - |
Brevibacillus brevis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.3 | 8.5 | half-maximal activity at pH 6.3 and pH 8.5 | Brevibacillus brevis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Brevibacillus brevis | |
NADH | - |
Brevibacillus brevis | |
NADPH | 2% activity of NADH | Brevibacillus brevis |