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Literature summary for 1.14.99.39 extracted from

  • Zhang, S.; Li, W.; Zhang, D.; Huang, X.; Qin, W.; Gu, J.
    Purification and characterization of a low-temperature ammonia monooxygenase from heterotrophic nitrifier Acinetobacter sp. Y16 (2013), Desalin. Water Treatment, 53, 257-262.
No PubMed abstract available
Search for more literature for 1.14.99.39

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound Acinetobacter sp. 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
CuCl2 stimulates enzyme activity in vitro Acinetobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 1 * 31000, SDS-PAGE Acinetobacter sp.

Organism

Organism UniProt Comment Textmining
Acinetobacter sp.
-
-
-
Acinetobacter sp. Y16
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by anion exchange chromatography and gel filtration Acinetobacter sp.

Storage Stability

Storage Stability Organism
10°C, purified native enzyme, 2 days, stable Acinetobacter sp.
15°C, purified native enzyme, 1 day, stable Acinetobacter sp.
4°C, purified native enzyme, 5 days, stable Acinetobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme can catalyze the oxidation of ammonium without stabilizing agents in vitro at low temperatures Acinetobacter sp. ?
-
 ?
additional information the enzyme can catalyze the oxidation of ammonium without stabilizing agents in vitro at low temperatures Acinetobacter sp. Y16 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 31000, SDS-PAGE Acinetobacter sp.

Synonyms

Synonyms Comment Organism
AMO
-
 Acinetobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
15
-
 the enzyme can catalyze the oxidation of ammonium without stabilizing agents in vitro at low temperatures Acinetobacter sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4 20 activity range, inactive below and above Acinetobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 15 purified native enzyme, stable, over 91% remaining activity Acinetobacter sp.
20
-
 purified native enzyme, inactivation Acinetobacter sp.

General Information

General Information Comment Organism
additional information the enzyme can catalyze the oxidation of ammonium without stabilizing agents in vitro at low temperatures Acinetobacter sp.