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Literature summary for 1.15.1.1 extracted from
- Three extremely thermostable proteins from Sulfolobuc and a reappraisal of the 'traffic rules' (1996), Biol. Chem., 377, 505-512.
General Stability
| General Stability | Organism |
|---|---|
| incubation with proteases (e.g. trypsin, V8 Staphylococcus aureus protease) has no effect on this enzyme | Sulfolobus acidocaldarius |
| the purified enzyme is stable in 6 M guanidinium chloride for 72 h loosing only 33% activity | Sulfolobus acidocaldarius |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no inhibition of the purified enzyme by 6 M guanidinium chloride or by proteases trypsin and Staphylococcus aureus V8 protease | Sulfolobus acidocaldarius |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Sulfolobus acidocaldarius | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24270 | - |
2 * 24270, sequence calculation | Sulfolobus acidocaldarius |
| 24270 | - |
2 * 24270, calculated from sequence | Sulfolobus acidocaldarius |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| guanidine-HCl | 6 M, incubation for 72 h, 67% of the initial activity is retained | Sulfolobus acidocaldarius |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfolobus acidocaldarius | - |
strain DSM 639, gene adk | - |
| Sulfolobus acidocaldarius | Q08713 | - |
- |
| Sulfolobus acidocaldarius DSM 639 | Q08713 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sulfolobus acidocaldarius |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | the organism has a very high enzyme content | Sulfolobus acidocaldarius | - |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 24270, sequence calculation | Sulfolobus acidocaldarius |
| dimer | 2 * 24270, calculated from sequence | Sulfolobus acidocaldarius |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SOD | - |
Sulfolobus acidocaldarius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
stable, the inaccessibility of a direct activity assay also excludes determination of a pH- or temperature-optimum | Sulfolobus acidocaldarius |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
due to its extraordinary heat stability, unfolding dynamics of this protein cannot be investigated by conventional physical methods below 100°C | Sulfolobus acidocaldarius |
| 95 | - |
purified enzyme, half-life is 48 h or above | Sulfolobus acidocaldarius |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
the inaccessibility of a direct activity assay also excludes determination of a pH- or temperature-optimum | Sulfolobus acidocaldarius |
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Release 2023.1 (January 2023)
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