Cloned (Comment) | Organism |
---|---|
gene Fe-SOD, DNA and amino acid sequence deteremination and analysis, sequence comparisons, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) cytosol, expression as thioredoxin-fusion protein in Escherichia coli | Jatropha curcas |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | gradual inhibition by increasing concentration of 2-mercaptoethanol, 20% inhibition at 2 mM, 36% at 16 mM | Jatropha curcas | |
EDTA | gradual inhibition by increasing concentration of EDTA | Jatropha curcas | |
guanidinium hydrochloride | 69% inhibition at 6 M | Jatropha curcas | |
H2O2 | gradual inhibition by increasing concentration of H2O2, 36% inhibition at 2 mM, 81% at 16 mM | Jatropha curcas |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | Fe-SOD, 0.41 atom of Fe per SOD subunit | Jatropha curcas | |
additional information | the enzyme contains no copper, zinc, or manganese | Jatropha curcas |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34589 | - |
2 * 34589, sequence calculation, 2 * 35000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 34652, recombinant His-tagged enzyme, mass spectrometry, 2 * 53000, recombinant thioredoxin-fusion enzyme, SDS-PAGE | Jatropha curcas |
34652 | - |
2 * 34589, sequence calculation, 2 * 35000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 34652, recombinant His-tagged enzyme, mass spectrometry, 2 * 53000, recombinant thioredoxin-fusion enzyme, SDS-PAGE | Jatropha curcas |
35000 | - |
2 * 34589, sequence calculation, 2 * 35000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 34652, recombinant His-tagged enzyme, mass spectrometry, 2 * 53000, recombinant thioredoxin-fusion enzyme, SDS-PAGE | Jatropha curcas |
53000 | - |
2 * 34589, sequence calculation, 2 * 35000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 34652, recombinant His-tagged enzyme, mass spectrometry, 2 * 53000, recombinant thioredoxin-fusion enzyme, SDS-PAGE | Jatropha curcas |
69000 | - |
recombinant His-tagged enzyme, gel filtration | Jatropha curcas |
69130 | - |
recombinant His-tagged enzyme, mass spectrometry | Jatropha curcas |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Jatropha curcas | H8YHP4 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme 25fold from Escherichia coli strain BL21(DE3) by immobilized metal-ion affinity and ion exchange chromatography, and isoelectric focusing to over 95% purity | Jatropha curcas |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
63.4 | - |
purified recombinant His-tagged enzyme, pH 7.5, 25°C | Jatropha curcas |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 34589, sequence calculation, 2 * 35000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 34652, recombinant His-tagged enzyme, mass spectrometry, 2 * 53000, recombinant thioredoxin-fusion enzyme, SDS-PAGE | Jatropha curcas |
Synonyms | Comment | Organism |
---|---|---|
Fe-SOD | - |
Jatropha curcas |
iron superoxide dismutase | - |
Jatropha curcas |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Jatropha curcas |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 37 | purified recombinant His-tagged enzyme, 60 min, stable | Jatropha curcas |
50 | 60 | purified recombinant His-tagged enzyme, 60 min, over 80% activity remaining | Jatropha curcas |
70 | - |
purified recombinant His-tagged enzyme, 60 min, 54% activity remaining | Jatropha curcas |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Jatropha curcas |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.5 | purified recombinant His-tagged enzyme, 25°C, 1 h, good stability | Jatropha curcas |