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Literature summary for 1.15.1.1 extracted from
- Cloning, overexpression, purification, and characterization of a new iron superoxide dismutase from Jatropha curcas (2013), Biotechnol. Appl. Biochem., 59, 338-345.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Fe-SOD, DNA and amino acid sequence deteremination and analysis, sequence comparisons, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) cytosol, expression as thioredoxin-fusion protein in Escherichia coli | Jatropha curcas |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | gradual inhibition by increasing concentration of 2-mercaptoethanol, 20% inhibition at 2 mM, 36% at 16 mM | Jatropha curcas |  |
| EDTA | gradual inhibition by increasing concentration of EDTA | Jatropha curcas | |
| guanidinium hydrochloride | 69% inhibition at 6 M | Jatropha curcas | |
| H2O2 | gradual inhibition by increasing concentration of H2O2, 36% inhibition at 2 mM, 81% at 16 mM | Jatropha curcas | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | Fe-SOD, 0.41 atom of Fe per SOD subunit | Jatropha curcas | |
| additional information | the enzyme contains no copper, zinc, or manganese | Jatropha curcas |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34589 | - |
2 * 34589, sequence calculation, 2 * 35000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 34652, recombinant His-tagged enzyme, mass spectrometry, 2 * 53000, recombinant thioredoxin-fusion enzyme, SDS-PAGE | Jatropha curcas |
| 34652 | - |
2 * 34589, sequence calculation, 2 * 35000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 34652, recombinant His-tagged enzyme, mass spectrometry, 2 * 53000, recombinant thioredoxin-fusion enzyme, SDS-PAGE | Jatropha curcas |
| 35000 | - |
2 * 34589, sequence calculation, 2 * 35000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 34652, recombinant His-tagged enzyme, mass spectrometry, 2 * 53000, recombinant thioredoxin-fusion enzyme, SDS-PAGE | Jatropha curcas |
| 53000 | - |
2 * 34589, sequence calculation, 2 * 35000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 34652, recombinant His-tagged enzyme, mass spectrometry, 2 * 53000, recombinant thioredoxin-fusion enzyme, SDS-PAGE | Jatropha curcas |
| 69000 | - |
recombinant His-tagged enzyme, gel filtration | Jatropha curcas |
| 69130 | - |
recombinant His-tagged enzyme, mass spectrometry | Jatropha curcas |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Jatropha curcas | H8YHP4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme 25fold from Escherichia coli strain BL21(DE3) by immobilized metal-ion affinity and ion exchange chromatography, and isoelectric focusing to over 95% purity | Jatropha curcas |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 63.4 | - |
purified recombinant His-tagged enzyme, pH 7.5, 25°C | Jatropha curcas |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 34589, sequence calculation, 2 * 35000, recombinant His-tagged enzyme, SDS-PAGE, 2 * 34652, recombinant His-tagged enzyme, mass spectrometry, 2 * 53000, recombinant thioredoxin-fusion enzyme, SDS-PAGE | Jatropha curcas |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Fe-SOD | - |
Jatropha curcas |
| iron superoxide dismutase | - |
Jatropha curcas |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Jatropha curcas |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 37 | purified recombinant His-tagged enzyme, 60 min, stable | Jatropha curcas |
| 50 | 60 | purified recombinant His-tagged enzyme, 60 min, over 80% activity remaining | Jatropha curcas |
| 70 | - |
purified recombinant His-tagged enzyme, 60 min, 54% activity remaining | Jatropha curcas |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Jatropha curcas |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9.5 | purified recombinant His-tagged enzyme, 25°C, 1 h, good stability | Jatropha curcas |
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