Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Aeropyrum pernix |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | inactivates the Fe-reconstituted SOD in a time-dependent manner, but not the Mn-reconstituted enzyme. The incubation time for 50% inactivation of the Fe-reconstituted enzyme in the presence of 0.24 mM H2O2 is 50 min | Aeropyrum pernix | |
Sodium azide | 50% inhibition of the Fe-reconstituted enzyme at 41 mM. Sodium azide does not inhibit the Mn-reconstituted superoxide dismutase even at concentrations up to 400 mM | Aeropyrum pernix | |
Sodium fluoride | inhibits both the Mn- and Fe-reconstituted enzyme. The concentrations of sodium fluoride causing 50% inhibition of the Mn- and Fe reconstituted enzymes are 89 and 13 mM, respectively | Aeropyrum pernix |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the enzyme contains both Mn and Fe. It is cambialistic, i.e. active with either Fe2+ or Mn2+. The specific activities were 906 U/mg with Mn2+ and 175 U/mg with Fe2+ | Aeropyrum pernix | |
Mn2+ | the enzyme contains both Mn and Fe. It is cambialistic, i.e. active with either Fe2+ or Mn2+. The specific activities were 906 U/mg with Mn2+ and 175 U/mg with Fe2+ | Aeropyrum pernix |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24600 | - |
2 * 24600, calculated from sequence | Aeropyrum pernix |
60000 | - |
gel filtration | Aeropyrum pernix |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum pernix | Q9Y8H8 | - |
- |
Aeropyrum pernix DSM 11879 | Q9Y8H8 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Aeropyrum pernix |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 24600, calculated from sequence | Aeropyrum pernix |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
105 | - |
5 h, no loss of activity | Aeropyrum pernix |
110 | - |
5 h, 44% loss of activity | Aeropyrum pernix |