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Literature summary for 1.15.1.1 extracted from

  • Wang, F.; Wu, Q.; Zhang, Z.; Chen, S.; Zhou, R.
    Cloning, expression, and characterization of iron superoxide dismutase in Sonneratia alba, a highly salt tolerant mangrove tree (2013), Protein J., 32, 259-265.
    View publication on PubMed
Search for more literature for 1.15.1.1

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative real-time PCR enzyme expresion analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta-gami Sonneratia alba

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ a Fe-SOD Sonneratia alba

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 x * 25000, recombinant His-tagged enzyme, SDS-PAGE Sonneratia alba

Organism

Organism UniProt Comment Textmining
Sonneratia alba
-
 collected from Qinglangang Mangrove Reserve, Wenchang, Hainan, China
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain Rosetta-gami by nickel affinity chromatography Sonneratia alba

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Sonneratia alba
-
fruit high expression level Sonneratia alba
-
leaf high expression level Sonneratia alba
-
additional information quantitative real-time PCR enzyme expresion analysis Sonneratia alba
-
root low expression level Sonneratia alba
-
seedling
-
 Sonneratia alba
-
stem
-
 Sonneratia alba
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme activity is measured byy the enzyme caused inhibition of the xanthine oxidase coupling reaction Sonneratia alba ?
-
 ?

Subunits

Subunits Comment Organism
? x * 25000, recombinant His-tagged enzyme, SDS-PAGE Sonneratia alba

Synonyms

Synonyms Comment Organism
FeSOD
-
 Sonneratia alba
iron superoxide dismutase
-
 Sonneratia alba

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Sonneratia alba

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 purified recombinant His-tagged enzyme, 1 h, 78% activity remaining Sonneratia alba

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Sonneratia alba

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 9.5 purified recombinant His-tagged enzyme, 25°C, 1 h, stable Sonneratia alba

pI Value

Organism Comment pI Value Maximum pI Value
Sonneratia alba sequence calculation
-
 6.42