Any feedback?
Literature summary for 1.15.1.1 extracted from
- Cloning, expression, and purification of a Cu/Zn superoxide dismutase from Jatropha curcas (2013), Z. Naturforsch. C, 68, 60-69.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His6-and thioredoxin-tagged enzyme in Escherichia coli | Jatropha curcas |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 8 mM, slight inhibition | Jatropha curcas |  |
| EDTA | at 16 mM | Jatropha curcas | |
| guanidinium hydrochloride | 6 M, slight inhibition | Jatropha curcas | |
| H2O2 | at 16 mM | Jatropha curcas | |
| imidazole | 1 mM, slight inhibition | Jatropha curcas | |
| Urea | 8 M, slight inhibition | Jatropha curcas | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | a Cu/Zn-SOD, the enzyme contains 1.54 mg/l copper atoms, and 0.239 mol Cu2+ per mol of enzyme | Jatropha curcas | |
| Zn2+ | a Cu/Zn-SOD, the enzyme contains 1.71 mg/l zinc atoms, 0.258 mol Zn2+ per mol of enzyme | Jatropha curcas | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16385 | - |
1 * 16385, sequence calculation, 1 * 16400, about, recombinant His6-and thioredoxin-tagged enzyme, mass spectrometry and SDS-PAGE | Jatropha curcas |
| 16400 | - |
about, recombinant His6-and thioredoxin-tagged enzyme, mass spectrometry | Jatropha curcas |
| 16500 | - |
recombinant His6-and thioredoxin-tagged enzyme, gel filtration | Jatropha curcas |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Jatropha curcas | H8YHP3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant His6-and thioredoxin-tagged enzyme from Escherichia coli, 12.2fold by two steps of nickel affinity chromatography and desalting gel filtration | Jatropha curcas |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 151.9 | - |
purified recombinant enzyme, pH and temperature not specified in the publication | Jatropha curcas |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 16385, sequence calculation, 1 * 16400, about, recombinant His6-and thioredoxin-tagged enzyme, mass spectrometry and SDS-PAGE | Jatropha curcas |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cu/Zn superoxide dismutase | - |
Jatropha curcas |
| Cu/Zn-SOD | - |
Jatropha curcas |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | about 80% activity remaining after 60 min | Jatropha curcas |
| 70 | - |
50% activity remaining after 30 min, inactivation after 60 min | Jatropha curcas |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Jatropha curcas | isoelectric focusing reveals four isoelectric variants in the recombinant JcCu/Zn-SOD, with pI values of 7.04, 7.33, 8.62, and 8.77 | - |
additional information |
| Jatropha curcas | isoelectric focusing, recombinant JcCu/Zn-SOD | - |
7.04 |
| Jatropha curcas | isoelectric focusing, recombinant JcCu/Zn-SOD | - |
7.33 |
| Jatropha curcas | isoelectric focusing, recombinant JcCu/Zn-SOD | - |
8.62 |
| Jatropha curcas | isoelectric focusing, recombinant JcCu/Zn-SOD | - |
8.77 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
