Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain BL21(DE3) | Megalodesulfovibrio gigas |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Zn-substituted rubredoxin | generation of Zn-substituted rubredoxin, which is diamagnetic and redox inactive, titration of Zn-rubredoxin with superoxide reductase, overview | Megalodesulfovibrio gigas |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics, overview | Megalodesulfovibrio gigas |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Megalodesulfovibrio gigas | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the class II enzyme has a single redox catalytic center consisting of an Fe atom bound to four nitrogen atoms from histidine side chains in the equatorial plane and to one cysteine sulfur in the axial plane | Megalodesulfovibrio gigas | |
additional information | ionic strength dependence of superoxide-mediated rubredoxin oxidation | Megalodesulfovibrio gigas |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
- |
Megalodesulfovibrio gigas |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced desulforedoxin + superoxide + 2 H+ | Megalodesulfovibrio gigas | - |
desulforedoxin + H2O2 | - |
? | |
reduced rubredoxin + superoxide + 2 H+ | Megalodesulfovibrio gigas | - |
rubredoxin + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Megalodesulfovibrio gigas | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) by ultracentrifugation, anion exchange chromatography, and gel filtration | Megalodesulfovibrio gigas |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced desulforedoxin + superoxide + 2 H+ | - |
Megalodesulfovibrio gigas | desulforedoxin + H2O2 | - |
? | |
reduced rubredoxin + superoxide + 2 H+ | - |
Megalodesulfovibrio gigas | rubredoxin + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Megalodesulfovibrio gigas |
Synonyms | Comment | Organism |
---|---|---|
SOR | - |
Megalodesulfovibrio gigas |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
ionic strength dependence of superoxide-mediated rubredoxin oxidation | Megalodesulfovibrio gigas |
7.6 | - |
assay at | Megalodesulfovibrio gigas |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
desulforedoxin | endogenous, contains Zn2+, the protein is able to transfer electrons to superoxide reductase with a maximum kapp of 31/min at an ionic strength of 57 mM, the enzyme complex with superoxide reductase is not detected by chemical shift perturbation | Megalodesulfovibrio gigas | |
additional information | titration of Zn-rubredoxin with superoxide reductase, overview. NMR competition assay between desulforedoxin and rubredoxin for binding to SOR, overview | Megalodesulfovibrio gigas | |
rubredoxin | endogenous. A monomeric, non-heme iron protein that contains a tetrahedral FeS4 metal center. The rubredoxin surface involved in the electron transfer complex with superoxide reductase comprises the solvent-exposed hydrophobic residues in the vicinity of its metal center, Cys9, Gly10, Cys42, Gly43, and Ala44. Kd of 0.003 mM at 59 mM ionic strength by NMR. Model structure of superoxide reductase-rubredoxin complex, overview | Megalodesulfovibrio gigas |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0004 | - |
pH 7.6, temperature not specified in the publication | Megalodesulfovibrio gigas | Zn-substituted rubredoxin |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the class II superoxide reductase family | Megalodesulfovibrio gigas |
additional information | model structure of SOR-rubredoxin complex, docking simulations, overview | Megalodesulfovibrio gigas |