Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00264 | - |
NADPH | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Homo sapiens | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
77000 | - |
gel filtration | Homo sapiens |
78000 | - |
1 * 78000, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.56 | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[methionine synthase]-cob(II)alamin + NADH + S-adenosyl-L-methionine | - |
Homo sapiens | [methionine synthase]methylcob(I)alamin + S-adenosylhomocysteine + NAD+ | - |
? | |
[Methionine synthase]-cob(II)alamin + NADPH + S-adenosyl-L-methionine | - |
Homo sapiens | [Methionine synthase]methylcob(I)alamin + S-adenosylhomocysteine + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 78000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
Methionine synthase reductase | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | dual flavoprotein with an equimolar concentration of FAD, 0.9 mol per mol of enzyme, and FMN, 1.1 mol per mol of enzyme | Homo sapiens | |
FMN | dual flavoprotein with an equimolar concentration of FAD, 0.9 mol per mol of enzyme, and FMN, 1.1 mol per mol of enzyme | Homo sapiens | |
NADH | can replace NADPH but only at significantly higher and nonphysiological concentrations | Homo sapiens | |
NADPH | preferred electron donor | Homo sapiens |