Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
K55A | the mutant shows strongly reduced activity compared to the wild type enzyme | Escherichia coli |
R130A | the mutant shows strongly reduced activity compared to the wild type enzyme | Escherichia coli |
R246A | the variant is rather unstable, showing precipitate formation and cofactor release during protein concentration | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
Fe(III)-enterobactin | wild type enzyme, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C | Escherichia coli | |
0.0014 | - |
Fe(III)-vibriobactin | wild type enzyme, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C | Escherichia coli | |
0.0018 | - |
Fe(III)-(N-2,3-dihydroxybenzoyl-L-serine)3 | wild type enzyme, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C | Escherichia coli | |
0.0042 | - |
Fe(III)-(N-2,3-dihydroxybenzoyl-Gly-Thr)3 | wild type enzyme, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C | Escherichia coli | |
0.0059 | - |
Fe(III)-enterobactin | mutant enzyme R130A, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C | Escherichia coli | |
0.0078 | - |
Fe(III)-enterobactin | mutant enzyme K55A, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C | Escherichia coli | |
0.0134 | - |
Fe(III)-dicitrate | wild type enzyme, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C | Escherichia coli | |
0.036 | - |
Fe(III)-(N-2,3-dihydroxybenzoyl-L-serine)3 | mutant enzyme R130A in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C | Escherichia coli | |
0.04 | - |
Fe(III)-(N-2,3-dihydroxybenzoyl-L-serine)3 | mutant enzyme K55A, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C | Escherichia coli | |
0.048 | - |
Fe(III)-aerobactin | wild type enzyme, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C | Escherichia coli | |
0.051 | - |
Fe(III)-dicitrate | mutant enzyme K55A, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C | Escherichia coli | |
0.066 | - |
Fe(III)-dicitrate | mutant enzyme R130A, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Strep-Tactin column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fe(III)-(N-2,3-dihydroxybenzoyl-Gly-Thr)3 + NADPH + H+ | - |
Escherichia coli | Fe(II) + (2,3-dihydroxybenzoyl-Gly-Thr)3 + NADP+ | - |
? | |
Fe(III)-(N-2,3-dihydroxybenzoyl-L-serine)3 + NADPH + H+ | - |
Escherichia coli | Fe(II) + (2,3-dihydroxybenzoyl-L-serine)3 + NADP+ | - |
? | |
Fe(III)-aerobactin + NADPH + H+ | - |
Escherichia coli | Fe(II) + aerobactin + NADP+ | - |
? | |
Fe(III)-bacillibactin + NADPH + H+ | - |
Escherichia coli | Fe(II) + bacillibacitin + NADP+ | - |
? | |
Fe(III)-dicitrate + NADPH + H+ | - |
Escherichia coli | Fe(II) + citrate + NADP+ | - |
? | |
Fe(III)-enterobactin + NADPH + H+ | - |
Escherichia coli | Fe(II) + enterobactin + NADP+ | - |
? | |
Fe(III)-vibriobactin + NADPH + H+ | - |
Escherichia coli | Fe(II) + vibriobactin + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ferric siderophore reductase | - |
Escherichia coli |
YqjH | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Escherichia coli | |
NADPH | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | the absence of YqjH slows growth | Escherichia coli |
metabolism | YqjH represents a redox factor that enhances the efficiency of ferric iron assimilation during siderophore-dependent iron homeostasis and enhances siderophore utilization in different iron acquisition pathways, including assimilation of low-potential ferric substrates that are not reduced by common cellular cofactors | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
Fe(III)-aerobactin | wild type enzyme, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C | Escherichia coli | |
0.032 | - |
Fe(III)-enterobactin | mutant enzyme K55A, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C | Escherichia coli | |
0.076 | - |
Fe(III)-enterobactin | mutant enzyme R130A, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C | Escherichia coli | |
0.091 | - |
Fe(III)-(N-2,3-dihydroxybenzoyl-L-serine)3 | mutant enzyme K55A, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C | Escherichia coli | |
0.132 | - |
Fe(III)-(N-2,3-dihydroxybenzoyl-L-serine)3 | mutant enzyme R130A in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C | Escherichia coli | |
0.139 | - |
Fe(III)-dicitrate | mutant enzyme R130A, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C | Escherichia coli | |
0.271 | - |
Fe(III)-dicitrate | mutant enzyme K55A, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C | Escherichia coli | |
1.268 | - |
Fe(III)-vibriobactin | wild type enzyme, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C | Escherichia coli | |
5.427 | - |
Fe(III)-dicitrate | wild type enzyme, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C | Escherichia coli | |
8.211 | - |
Fe(III)-enterobactin | wild type enzyme, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C | Escherichia coli | |
9.193 | - |
Fe(III)-(N-2,3-dihydroxybenzoyl-Gly-Thr)3 | wild type enzyme, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C | Escherichia coli | |
23.35 | - |
Fe(III)-(N-2,3-dihydroxybenzoyl-L-serine)3 | wild type enzyme, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C | Escherichia coli |