Cloned (Comment) | Organism |
---|---|
expressed in expressed in baby hamster kidney cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D616A/H621A/E960A/H965A | iron binding sites are mutated but one is unaffected. Km and kcat for substrate 4-phenylenediamine decreased compared to wild-type. kcat (Fe2+) up to 10fold decreased compared to wild-type. Mutant does not retain a high-affinity iron oxidation component | Homo sapiens |
E264A/H269A/D616A/H621A | iron binding sites are mutated but one is unaffected. Km and kcat for substrate 4-phenylenediamine decreased compared to wild-type. Km for high-affinity oxidation of Fe2+ decreased compared to wild-type. kcat (Fe2+) up to 10fold decreased compared to wild-type. Only mutant that retains a high-affinity iron oxidation component | Homo sapiens |
E264A/H269A/D616A/H621A/E960A/H965A | all three iron binding sites are mutated. Km and kcat for substrate 4-phenylenediamine decreased compared to wild-type. kcat (Fe2+) up to 75 fold decreased compared to wild-type. Mutant does not retain a high-affinity iron oxidation component | Homo sapiens |
E264A/H269A/E960A/H965A | iron binding sites are mutated but one is unaffected. Km and kcat for substrate 4-phenylenediamine decreased compared to wild-type. kcat (Fe2+) up to 10fold decreased compared to wild-type. Mutant does not retain a high-affinity iron oxidation component | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
4-phenylenediamine | pH 5, 20°C | Homo sapiens | |
0.0035 | - |
Fe2+ | pH 5, 20°C, Km for high-affinity iron-binding sites using a two-component Eadie-Hofstee plot, Vmax: 67 nmol/min | Homo sapiens | |
0.004 | - |
Fe2+ | pH 5, 20°C | Homo sapiens | |
0.0107 | - |
Fe2+ | pH 5, 20°C, Km for low-affinity iron-binding sites using a two-component Eadie-Hofstee plot, Vmax: 286 nmol/min | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | rhHp possesses both high-affinity and low-affinity binding sites for ferrous iron | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-phenylenediamine + Fe2+ + O2 | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
hephaestin | - |
Homo sapiens |
rhHp | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
Fe2+ | pH 5, 20°C | Homo sapiens | |
0.48 | - |
4-phenylenediamine | pH 5, 20°C | Homo sapiens |