Cloned (Comment) | Organism |
---|---|
recombinantly expressed in Sf9 cells using the baculovirus expression system. Recombinant MCO1 is expressed without the von Willebrand factor domains and transmembrane segment, and Arg454 is mutated to alanine to reduce proteolytic cleavage | Drosophila melanogaster |
Protein Variants | Comment | Organism |
---|---|---|
R454A | Arg454 is mutated to Ala in order to reduce proteolytic cleavage | Drosophila melanogaster |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using concanavalin-A-Sepharose and Q-sepharose | Drosophila melanogaster |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
malpighian tubule | - |
Drosophila melanogaster | - |
midgut | basal surface of midgut | Drosophila melanogaster | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
MCO1 has ferroxidase activity | Drosophila melanogaster |
Synonyms | Comment | Organism |
---|---|---|
MCO1 | - |
Drosophila melanogaster |
multicopper oxidase-1 | - |
Drosophila melanogaster |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Drosophila melanogaster |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | highest activity | Drosophila melanogaster |
General Information | Comment | Organism |
---|---|---|
malfunction | knockdown of MCO1 is correlated with increased longevity on high-iron food and decreased iron accumulation | Drosophila melanogaster |