Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain BL21(DE3) | Arabidopsis thaliana |
expression in Escherichia coli strain DH5alpha, expression as MBT-fusion protein followed by cleavage of the protein tag by Factor Xa protease | Medicago truncatula |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(+)-catechin | - |
Arabidopsis thaliana | |
cyanidin | substrate inhibition at high concentration | Arabidopsis thaliana | |
dihydroquercetin | - |
Arabidopsis thaliana | |
additional information | no inhibition by (+)-catechin and dihydroquercetin | Medicago truncatula | |
Na+ | above 200 mM | Medicago truncatula | |
pelargonidin | substrate inhibition at high concentration | Arabidopsis thaliana | |
quercetin | - |
Arabidopsis thaliana | |
quercetin | - |
Medicago truncatula |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Arabidopsis thaliana | |
additional information | - |
additional information | enzyme shows typical Michaelis-Menten kinetics for each substrate | Medicago truncatula |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme is not affected by Na+ at up to 400 mM | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyanidin + NADPH + H+ | Arabidopsis thaliana | - |
(-)-epicatechin + NADP+ + H2O | i.e. (2R,3R)-3-cis-flavan-3-ol | ? | |
cyanidin + NADPH + H+ | Medicago truncatula | - |
(-)-epicatechin + NADP+ + H2O | i.e. (2R,3R)-3-cis-flavan-3-ol | ? | |
additional information | Arabidopsis thaliana | enzyme is involved in the biosynthesis of condensed tannins in the flavonoid pathway converting anthocyanidins into 2,3-cis-flavan-3-ols, overview | ? | - |
? | |
additional information | Medicago truncatula | enzyme is involved in the biosynthesis of condensed tannins in the flavonoid pathway converting anthocyanidins into 2,3-cis-flavan-3-ols, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
cv. Columbia, gene BANYULS | - |
Medicago truncatula | - |
gene BANYULS | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli | Arabidopsis thaliana |
recombinant enzyme from Escherichia coli | Medicago truncatula |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyanidin + NADPH + H+ | - |
Arabidopsis thaliana | (-)-epicatechin + NADP+ + H2O | i.e. (2R,3R)-3-cis-flavan-3-ol | ? | |
cyanidin + NADPH + H+ | - |
Medicago truncatula | (-)-epicatechin + NADP+ + H2O | i.e. (2R,3R)-3-cis-flavan-3-ol | ? | |
cyanidin + NADPH + H+ | - |
Arabidopsis thaliana | (-)-epicatechin + (-)-catechin + NADP+ + H2O | (-)-epicatechin, i.e. (2R,3R)-3-cis-flavan-3-ol, is the major product, formation of (-)-catechin by nonenzymatic epimerization is possible | ? | |
cyanidin + NADPH + H+ | - |
Medicago truncatula | (-)-epicatechin + (-)-catechin + NADP+ + H2O | (-)-epicatechin, i.e. (2R,3R)-3-cis-flavan-3-ol, is the major product, formation of (-)-catechin by nonenzymatic epimerization is possible | ? | |
delphinidin + NADPH | - |
Arabidopsis thaliana | (-)-epigallocatechin + (-)-gallocatechin + NADP+ + H2O | (-)-epigallocatechin is the major product, formation of (-)-gallocatechin by nonenzymatic epimerization is possible | ? | |
delphinidin + NADPH | - |
Medicago truncatula | (-)-epigallocatechin + (-)-gallocatechin + NADP+ + H2O | (-)-epigallocatechin is the major product, formation of (-)-gallocatechin by nonenzymatic epimerization is possible | ? | |
additional information | enzyme is involved in the biosynthesis of condensed tannins in the flavonoid pathway converting anthocyanidins into 2,3-cis-flavan-3-ols, overview | Arabidopsis thaliana | ? | - |
? | |
additional information | enzyme is involved in the biosynthesis of condensed tannins in the flavonoid pathway converting anthocyanidins into 2,3-cis-flavan-3-ols, overview | Medicago truncatula | ? | - |
? | |
additional information | substrate preference in descending order: cyanidin, pelargonidin, delphinidin | Medicago truncatula | ? | - |
? | |
additional information | substrate preference in descending order: delphinidin, pelargonidin, cyanidin | Arabidopsis thaliana | ? | - |
? | |
pelargonidin + NADPH | - |
Arabidopsis thaliana | (-)-epiafzelechin + (-)-afzelechin + NADP+ + H2O | (-)-epiafzelechin is the major product, formation of (-)-afzelechin by nonenzymatic epimerization is possible | ? | |
pelargonidin + NADPH | - |
Medicago truncatula | (-)-epiafzelechin + (-)-afzelechin + NADP+ + H2O | (-)-epiafzelechin is the major product, formation of (-)-afzelechin by nonenzymatic epimerization is possible | ? |
Synonyms | Comment | Organism |
---|---|---|
ANR | - |
Arabidopsis thaliana |
ANR | - |
Medicago truncatula |
anthocyanidin reductase | - |
Arabidopsis thaliana |
anthocyanidin reductase | - |
Medicago truncatula |
BAN | - |
Arabidopsis thaliana |
BAN | - |
Medicago truncatula |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Medicago truncatula |
55 | - |
- |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6 | depending on the buffer system used | Medicago truncatula |
7 | - |
assay at | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Medicago truncatula | |
NADPH | slight preference for | Medicago truncatula | |
NADPH | specific for, positive cooperativity | Arabidopsis thaliana |