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Literature summary for 1.17.1.3 extracted from

  • Xie, D.Y.; Sharma, S.B.; Dixon, R.A.
    Anthocyanidin reductases from Medicago truncatula and Arabidopsis thaliana (2004), Arch. Biochem. Biophys., 422, 91-102.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain BL21(DE3) Arabidopsis thaliana
expression in Escherichia coli strain DH5alpha, expression as MBT-fusion protein followed by cleavage of the protein tag by Factor Xa protease Medicago truncatula

Inhibitors

Inhibitors Comment Organism Structure
(+)-catechin
-
Arabidopsis thaliana
cyanidin substrate inhibition at high concentration Arabidopsis thaliana
dihydroquercetin
-
Arabidopsis thaliana
additional information no inhibition by (+)-catechin and dihydroquercetin Medicago truncatula
Na+ above 200 mM Medicago truncatula
pelargonidin substrate inhibition at high concentration Arabidopsis thaliana
quercetin
-
Arabidopsis thaliana
quercetin
-
Medicago truncatula

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Arabidopsis thaliana
additional information
-
additional information enzyme shows typical Michaelis-Menten kinetics for each substrate Medicago truncatula

Metals/Ions

Metals/Ions Comment Organism Structure
additional information enzyme is not affected by Na+ at up to 400 mM Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cyanidin + NADPH + H+ Arabidopsis thaliana
-
(-)-epicatechin + NADP+ + H2O i.e. (2R,3R)-3-cis-flavan-3-ol ?
cyanidin + NADPH + H+ Medicago truncatula
-
(-)-epicatechin + NADP+ + H2O i.e. (2R,3R)-3-cis-flavan-3-ol ?
additional information Arabidopsis thaliana enzyme is involved in the biosynthesis of condensed tannins in the flavonoid pathway converting anthocyanidins into 2,3-cis-flavan-3-ols, overview ?
-
?
additional information Medicago truncatula enzyme is involved in the biosynthesis of condensed tannins in the flavonoid pathway converting anthocyanidins into 2,3-cis-flavan-3-ols, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
cv. Columbia, gene BANYULS
-
Medicago truncatula
-
gene BANYULS
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli Arabidopsis thaliana
recombinant enzyme from Escherichia coli Medicago truncatula

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyanidin + NADPH + H+
-
Arabidopsis thaliana (-)-epicatechin + NADP+ + H2O i.e. (2R,3R)-3-cis-flavan-3-ol ?
cyanidin + NADPH + H+
-
Medicago truncatula (-)-epicatechin + NADP+ + H2O i.e. (2R,3R)-3-cis-flavan-3-ol ?
cyanidin + NADPH + H+
-
Arabidopsis thaliana (-)-epicatechin + (-)-catechin + NADP+ + H2O (-)-epicatechin, i.e. (2R,3R)-3-cis-flavan-3-ol, is the major product, formation of (-)-catechin by nonenzymatic epimerization is possible ?
cyanidin + NADPH + H+
-
Medicago truncatula (-)-epicatechin + (-)-catechin + NADP+ + H2O (-)-epicatechin, i.e. (2R,3R)-3-cis-flavan-3-ol, is the major product, formation of (-)-catechin by nonenzymatic epimerization is possible ?
delphinidin + NADPH
-
Arabidopsis thaliana (-)-epigallocatechin + (-)-gallocatechin + NADP+ + H2O (-)-epigallocatechin is the major product, formation of (-)-gallocatechin by nonenzymatic epimerization is possible ?
delphinidin + NADPH
-
Medicago truncatula (-)-epigallocatechin + (-)-gallocatechin + NADP+ + H2O (-)-epigallocatechin is the major product, formation of (-)-gallocatechin by nonenzymatic epimerization is possible ?
additional information enzyme is involved in the biosynthesis of condensed tannins in the flavonoid pathway converting anthocyanidins into 2,3-cis-flavan-3-ols, overview Arabidopsis thaliana ?
-
?
additional information enzyme is involved in the biosynthesis of condensed tannins in the flavonoid pathway converting anthocyanidins into 2,3-cis-flavan-3-ols, overview Medicago truncatula ?
-
?
additional information substrate preference in descending order: cyanidin, pelargonidin, delphinidin Medicago truncatula ?
-
?
additional information substrate preference in descending order: delphinidin, pelargonidin, cyanidin Arabidopsis thaliana ?
-
?
pelargonidin + NADPH
-
Arabidopsis thaliana (-)-epiafzelechin + (-)-afzelechin + NADP+ + H2O (-)-epiafzelechin is the major product, formation of (-)-afzelechin by nonenzymatic epimerization is possible ?
pelargonidin + NADPH
-
Medicago truncatula (-)-epiafzelechin + (-)-afzelechin + NADP+ + H2O (-)-epiafzelechin is the major product, formation of (-)-afzelechin by nonenzymatic epimerization is possible ?

Synonyms

Synonyms Comment Organism
ANR
-
Arabidopsis thaliana
ANR
-
Medicago truncatula
anthocyanidin reductase
-
Arabidopsis thaliana
anthocyanidin reductase
-
Medicago truncatula
BAN
-
Arabidopsis thaliana
BAN
-
Medicago truncatula

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Medicago truncatula
55
-
-
Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6 depending on the buffer system used Medicago truncatula
7
-
assay at Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NADH
-
Medicago truncatula
NADPH slight preference for Medicago truncatula
NADPH specific for, positive cooperativity Arabidopsis thaliana