Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | contains FeSI and FeSII clusters | Pseudomonas putida | |
Iron-sulfur-center | - |
Pseudomonas putida | |
Molybdenum | contains molybdenum-molybdopterin | Pseudomonas putida | |
molybdenum-center | - |
Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46238 | - |
2 * 91041 + 2 * 46238, MALDI-TOF mass spectrometry | Pseudomonas putida |
51000 | - |
2 * 94000 + 2 * 51000, SDS-PAGE | Pseudomonas putida |
91041 | - |
2 * 91041 + 2 * 46238, MALDI-TOF mass spectrometry | Pseudomonas putida |
94000 | - |
2 * 94000 + 2 * 51000, SDS-PAGE | Pseudomonas putida |
580000 | - |
SDS-PAGE | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hypoxanthine + NAD+ + H2O | Pseudomonas putida | - |
xanthine + NADH + H+ | - |
? | |
xanthine + NAD+ + H2O | Pseudomonas putida | - |
urate + NADH | - |
? | |
xanthine + O2 + H2O | Pseudomonas putida | 4% of the activity compared to xanthine-NAD+ | urate + O2- + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida 86 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
ammonium sulfate precipitation, phenyl-Sepharose column chromatography, DEAE-10 Macro-Prep MP10 column chromatography, and Bio-Prep SE-1000/17 column chromatography | Pseudomonas putida |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | inducible by hypoxanthine | Pseudomonas putida | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.9 | - |
crude extract | Pseudomonas putida |
26.7 | - |
hypoxanthine-NAD+ assay | Pseudomonas putida |
35.2 | - |
after 39.1fold purification | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxybenzaldehyde + NAD+ + H2O | 1.2% activity compared to xanthine | Pseudomonas putida | 3,4-dihydroxybenzoate + NADH + H+ | - |
? | |
3,4-dihydroxybenzaldehyde + NAD+ + H2O | 1.2% activity compared to xanthine | Pseudomonas putida 86 | 3,4-dihydroxybenzoate + NADH + H+ | - |
? | |
4-dimethylaminobenzaldehyde + NAD+ + H2O | 0.3% activity compared to xanthine | Pseudomonas putida | 4-dimethylaminobenzoate + NADH | - |
? | |
4-hydroxybenzaldehyde + NAD+ + H2O | 0.7% activity compared to xanthine | Pseudomonas putida | 4-hydroxybenzoate + NADH + H+ | - |
? | |
4-hydroxybenzaldehyde + NAD+ + H2O | 0.7% activity compared to xanthine | Pseudomonas putida 86 | 4-hydroxybenzoate + NADH + H+ | - |
? | |
benzaldehyde + NAD+ + H2O | 1.3% activity compared to xanthine | Pseudomonas putida | benzoate + NADH + H+ | - |
? | |
benzaldehyde + NAD+ + H2O | 1.3% activity compared to xanthine | Pseudomonas putida 86 | benzoate + NADH + H+ | - |
? | |
hypoxanthine + ferricyanide + H2O | - |
Pseudomonas putida | xanthine + ferrocyanide | - |
? | |
hypoxanthine + ferricyanide + H2O | - |
Pseudomonas putida 86 | xanthine + ferrocyanide | - |
? | |
hypoxanthine + NAD+ + H2O | - |
Pseudomonas putida | xanthine + NADH + H+ | - |
? | |
hypoxanthine + NAD+ + H2O | 149% activity compared to xanthine | Pseudomonas putida | urate + ? + NADH | - |
? | |
additional information | NAD+ is the most effcient electron acceptor, followed by 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl-2H-tetrazolium chloride and ferricyanide | Pseudomonas putida | ? | - |
? | |
additional information | NAD+ is the most effcient electron acceptor, followed by 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl-2H-tetrazolium chloride and ferricyanide | Pseudomonas putida 86 | ? | - |
? | |
phthalazine + NAD+ + H2O | 0.4% activity compared to xanthine | Pseudomonas putida | 1-(2H)-phthalazinone + NADH | - |
? | |
purine + NAD+ + H2O | 5% activity compared to xanthine | Pseudomonas putida | 8-hydroxypurine + NADH + H+ | - |
? | |
quinazoline + NAD+ + H2O | 2% activity compared to xanthine | Pseudomonas putida | 4-(3H)-quinazolinone + NADH | - |
? | |
xanthine + ferricyanide + H2O | 58% of the activity compared to xanthine-NAD+ | Pseudomonas putida | urate + ferrocyanide | - |
? | |
xanthine + NAD+ + H2O | - |
Pseudomonas putida | urate + NADH | - |
? | |
xanthine + NAD+ + H2O | 100% activity | Pseudomonas putida | urate + NADH | - |
? | |
xanthine + O2 + H2O | 4% of the activity compared to xanthine-NAD+ | Pseudomonas putida | urate + O2- + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterooctamer | 2 * 91041 + 2 * 46238, MALDI-TOF mass spectrometry | Pseudomonas putida |
heterooctamer | 2 * 94000 + 2 * 51000, SDS-PAGE | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
XDH | - |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Pseudomonas putida | |
additional information | cofactor compostion similar to eukaryotic enzymes | Pseudomonas putida | |
NAD+ | - |
Pseudomonas putida | |
NADP+ | - |
Pseudomonas putida |