General Stability | Organism |
---|---|
crude and purified enzyme preparations form readily insoluble inactive aggregates, not stable to freezing and thawing | Delftia acidovorans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenine | some non-competitive inhibition | Delftia acidovorans | |
guanine | some inhibition | Delftia acidovorans | |
purine | some inhibition | Delftia acidovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
xanthine | NAD+ | Delftia acidovorans | |
0.07 | - |
xanthine | cosubstrate | Delftia acidovorans | |
0.12 | - |
NAD+ | cosubstrate: xanthine | Delftia acidovorans | |
0.16 | - |
NAD+ | cosubstrate: hypoxanthine | Delftia acidovorans | |
0.29 | - |
hypoxanthine | cosubstrate: NAD+ | Delftia acidovorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
2 * 81000 + 2 * 63000, SDS-PAGE | Delftia acidovorans |
81000 | - |
2 * 81000 + 2 * 63000, SDS-PAGE | Delftia acidovorans |
275000 | - |
gel filtration | Delftia acidovorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hypoxanthine + NAD+ + H2O | Delftia acidovorans | - |
xanthine + NADH + H+ | - |
? | |
purine + NAD+ + H2O | Delftia acidovorans | poor substrate | ? + NADH | - |
? | |
xanthine + NAD+ + H2O | Delftia acidovorans | - |
urate + NADH | - |
? | |
xanthine + NADP+ + H2O | Delftia acidovorans | 11% of the activity compared to NAD+ | urate + NADPH | - |
? | |
xanthine + O2 + H2O | Delftia acidovorans | low activity | urate + O2- + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Delftia acidovorans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
near homogeneity, chromatography techniques | Delftia acidovorans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Delftia acidovorans | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hypoxanthine + NAD+ + H2O | - |
Delftia acidovorans | xanthine + NADH + H+ | - |
? | |
NADH + electron acceptor + H2O | extremely slow reoxidation rate | Delftia acidovorans | NAD+ + reduced electron acceptor | - |
? | |
purine + NAD+ + H2O | poor substrate | Delftia acidovorans | ? + NADH | - |
? | |
xanthine + NAD+ + H2O | - |
Delftia acidovorans | urate + NADH | - |
? | |
xanthine + NADP+ + H2O | 11% of the activity compared to NAD+ | Delftia acidovorans | urate + NADPH | - |
? | |
xanthine + O2 + H2O | low activity | Delftia acidovorans | urate + O2- + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 2 * 81000 + 2 * 63000, SDS-PAGE | Delftia acidovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
- |
Delftia acidovorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Delftia acidovorans | |
NADP+ | 11% of the activity compared to NAD+ | Delftia acidovorans |