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Literature summary for 1.17.1.4 extracted from

  • Sin, I.L.
    Purification and properties of xanthine dehydrogenase from Pseudomonas acidovorans (1975), Biochim. Biophys. Acta, 410, 12-20.
    View publication on PubMed

General Stability

General Stability Organism
crude and purified enzyme preparations form readily insoluble inactive aggregates, not stable to freezing and thawing Delftia acidovorans

Inhibitors

Inhibitors Comment Organism Structure
adenine some non-competitive inhibition Delftia acidovorans
guanine some inhibition Delftia acidovorans
purine some inhibition Delftia acidovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
xanthine NAD+ Delftia acidovorans
0.07
-
xanthine cosubstrate Delftia acidovorans
0.12
-
NAD+ cosubstrate: xanthine Delftia acidovorans
0.16
-
NAD+ cosubstrate: hypoxanthine Delftia acidovorans
0.29
-
hypoxanthine cosubstrate: NAD+ Delftia acidovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
2 * 81000 + 2 * 63000, SDS-PAGE Delftia acidovorans
81000
-
2 * 81000 + 2 * 63000, SDS-PAGE Delftia acidovorans
275000
-
gel filtration Delftia acidovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hypoxanthine + NAD+ + H2O Delftia acidovorans
-
xanthine + NADH + H+
-
?
purine + NAD+ + H2O Delftia acidovorans poor substrate ? + NADH
-
?
xanthine + NAD+ + H2O Delftia acidovorans
-
urate + NADH
-
?
xanthine + NADP+ + H2O Delftia acidovorans 11% of the activity compared to NAD+ urate + NADPH
-
?
xanthine + O2 + H2O Delftia acidovorans low activity urate + O2- + 2 H+
-
?

Organism

Organism UniProt Comment Textmining
Delftia acidovorans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
near homogeneity, chromatography techniques Delftia acidovorans

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Delftia acidovorans
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hypoxanthine + NAD+ + H2O
-
Delftia acidovorans xanthine + NADH + H+
-
?
NADH + electron acceptor + H2O extremely slow reoxidation rate Delftia acidovorans NAD+ + reduced electron acceptor
-
?
purine + NAD+ + H2O poor substrate Delftia acidovorans ? + NADH
-
?
xanthine + NAD+ + H2O
-
Delftia acidovorans urate + NADH
-
?
xanthine + NADP+ + H2O 11% of the activity compared to NAD+ Delftia acidovorans urate + NADPH
-
?
xanthine + O2 + H2O low activity Delftia acidovorans urate + O2- + 2 H+
-
?

Subunits

Subunits Comment Organism
tetramer 2 * 81000 + 2 * 63000, SDS-PAGE Delftia acidovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
-
Delftia acidovorans

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Delftia acidovorans
NADP+ 11% of the activity compared to NAD+ Delftia acidovorans