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Literature summary for 1.17.1.4 extracted from

  • Perez-Vicente, R.; Alamillo, J.M.; Cardenas, J.; Pineda, M.
    Purification and substrate inactivation of xanthine dehydrogenase from Chlamydomonas reinhardtii (1992), Biochim. Biophys. Acta, 1117, 159-166.
    View publication on PubMed
Search for more literature for 1.17.1.4

Inhibitors

Inhibitors Comment Organism Structure
8-Azaadenine competitive inhibition of xanthine oxidation, Ki: 0.25 mM Chlamydomonas reinhardtii
acetaldehyde inactivation Chlamydomonas reinhardtii
adenine competitive inhibition of xanthine oxidation, Ki: 0.13 mM Chlamydomonas reinhardtii
hypoxanthine inactivation of xanthine oxidase activity, not in the presence of NAD+ Chlamydomonas reinhardtii
NADH inactivation closely related to associated diaphorase activity Chlamydomonas reinhardtii
NADPH inactivation closely related to diaphorase activity Chlamydomonas reinhardtii
Sodium dithionite irreversible inactivation by reduction of xanthine dehydrogenase, no recovery after dithionite elimination Chlamydomonas reinhardtii
xanthine irreversible inactivation by reduction of xanthine dehydrogenase; irreversible inhibition of xanthine oxidase activity, adenine and 8-azaadenine protects against inactivation, ferricyanide partially protects against inactivation, no inactivation in the presence of NAD+ Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hypoxanthine + NAD+ + H2O Chlamydomonas reinhardtii
-
 xanthine + NADH + H+
-
 ?
hypoxanthine + NADP+ + H2O Chlamydomonas reinhardtii
-
 xanthine + NADPH
-
 ?
xanthine + NAD+ + H2O Chlamydomonas reinhardtii
-
 urate + NADH
-
 ?

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography, preparative gel electrophoresis Chlamydomonas reinhardtii

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Chlamydomonas reinhardtii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.69
-
-
 Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
8-azaxanthine + NAD+ + H2O very low activity Chlamydomonas reinhardtii 8-aza-urate + NADH
-
 ?
acetaldehyde + NAD+ + H2O very low activity Chlamydomonas reinhardtii acetate + NADH + H+
-
 ?
adenine + NAD+ + H2O low activity Chlamydomonas reinhardtii ? + NADH
-
 ?
hypoxanthine + NAD+ + H2O
-
 Chlamydomonas reinhardtii xanthine + NADH + H+
-
 ?
hypoxanthine + NADP+ + H2O
-
 Chlamydomonas reinhardtii xanthine + NADPH
-
 ?
NADH + electron acceptor + H2O electron acceptor: nitroblue tetrazolium Chlamydomonas reinhardtii NAD+ + reduced electron acceptor
-
 ?
NADPH + nitroblue tetrazolium + H2O diaphorase activity Chlamydomonas reinhardtii NADP+ + reduced nitroblue tetrazolium
-
 ?
xanthine + NAD+ + H2O
-
 Chlamydomonas reinhardtii urate + NADH
-
 ?

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Chlamydomonas reinhardtii
NADP+
-
 Chlamydomonas reinhardtii