Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.17.1.4 extracted from

  • Lyon, E.S.; Garrett, R.H.
    Regulation, purification, and properties of xanthine dehydrogenase in Neurospora crassa (1978), J. Biol. Chem., 253, 2604-2614.
    View publication on PubMed
Search for more literature for 1.17.1.4

Inhibitors

Inhibitors Comment Organism Structure
2-amino-4-hydroxypterin substrate inhibition above 0.01 mM Neurospora crassa
2-Iodosobenzoic acid effective inhibition of xanthine and pterine oxidation Neurospora crassa
4-hydroxymercuribenzoate effective inhibition of xanthine and pterine oxidation Neurospora crassa
4-hydroxypyrazolo(3,4-d)pyrimidine i.e. allopurinol; inhibition of pterine oxidation at 0.0003 mM Neurospora crassa
adenine effective inhibition of xanthine oxidation at 0.001 mM Neurospora crassa
arsenite inhibition of xanthine or pterine oxidation at 0.3 mM, diaphorase activity unaffected Neurospora crassa
guanine effective inhibition of xanthine oxidation at 0.001 mM Neurospora crassa
KCN 35% inhibition of pterine oxidation, 60% inhibition of diaphorase activity at 5 mM Neurospora crassa
NaN3 slight inhibition of dehydrogenase activity Neurospora crassa
o-phenanthroline 50% inhibition at 5-15 mM Neurospora crassa
Thiourea 65% inhibition of xanthine dehydrogenase activity at 20 mM Neurospora crassa
Urate inhibition of xanthine oxidation at 0.06 mM Neurospora crassa
xanthine substrate inhibition above 0.13 mM Neurospora crassa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0022
-
 NAD+ oxidation of 2-amino-4-hydroxy-pterin Neurospora crassa
0.0035
-
 2-amino-4-hydroxypterin
-
 Neurospora crassa
0.0082
-
 xanthine xanthine dehydrogenase activity Neurospora crassa
0.021
-
 hypoxanthine xanthine dehydrogenase activity Neurospora crassa
0.021
-
 2,6-dichloroindophenol diaphorase activity Neurospora crassa
0.028
-
 NAD+ xanthine dehydrogenase activity Neurospora crassa
6
-
 NADH diaphorase activity Neurospora crassa

Metals/Ions

Metals/Ions Comment Organism Structure
Fe 12.1 mol iron per mol enzyme Neurospora crassa
Mo 1 mol molybdenum per mol enzyme Neurospora crassa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
155000
-
 2 * 155000, SDS-PAGE Neurospora crassa
357000
-
 sucrose density gradient centrifugation Neurospora crassa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-amino-4-hydroxy-pterin + NAD+ + H2O Neurospora crassa i.e. pterin isoxanthopterin + NADH
-
 r
hypoxanthine + NAD+ + H+ + O2- + H2O Neurospora crassa
-
 xanthine + NADH + H2O2
-
 r
xanthine + NAD+ + H2O Neurospora crassa degradative pathway of conversion of purines to ammonia urate + NADH
-
 r

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, immunoabsorption chromatography Neurospora crassa

Source Tissue

Source Tissue Comment Organism Textmining
mycelium inducible Neurospora crassa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.152
-
-
 Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,6-dichloroindophenol + NADH + H+
-
 Neurospora crassa reduced 2,6-dichlorophenolindophenol + NAD+
-
 r
2-amino-4-hydroxy-pterin + NAD+ + H2O i.e. pterin Neurospora crassa isoxanthopterin + NADH
-
 r
2-amino-4-hydroxypterin + NAD+ + H2O i.e. pterin Neurospora crassa isoxanthopterin + NADH
-
 r
hypoxanthine + NAD+ + H+ + O2- + H2O
-
 Neurospora crassa xanthine + NADH + H2O2
-
 r
NADH + electron acceptor + H2O 2,6-dichloroindophenol as electron acceptor Neurospora crassa NAD+ + reduced electron acceptor
-
 ?
xanthine + NAD+ + H2O degradative pathway of conversion of purines to ammonia Neurospora crassa urate + NADH
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 155000, SDS-PAGE Neurospora crassa

Cofactor

Cofactor Comment Organism Structure
FAD 2 mol per mol enzyme Neurospora crassa
additional information non-heme flavoprotein Neurospora crassa
NAD+ cannot be replaced by NADP+ Neurospora crassa
NADH
-
 Neurospora crassa