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Literature summary for 1.17.1.5 extracted from

  • Hirschberg, R.; Ensign, J.C.
    Oxidation of nicotinic acid by a Bacillus species: purification and properties of nicotinic acid and 6-hydroxynicotinic acid hydroxylases (1971), J. Bacteriol., 108, 751-756.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 1 mM, 30-50% inhibition Bacillus sp. (in: Bacteria)
2,2'-dipyridyl 0.1 mM, 30-50% inhibition Bacillus sp. (in: Bacteria)
Atabrine 1 mM, 30-50% inhibition Bacillus sp. (in: Bacteria)
NaCl sufficient NaCl inhibits enzyme activity significantly Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
nicotinate
-
Bacillus sp. (in: Bacteria)

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Bacillus sp. (in: Bacteria)
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
400000
-
gel filtration Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nicotinate + H2O + electron acceptor Bacillus sp. (in: Bacteria) first step of nicotinate catabolism 6-hydroxynicotinate + reduced electron acceptor
-
?

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-

Purification (Commentary)

Purification (Comment) Organism
24.4fold purification Bacillus sp. (in: Bacteria)

Renatured (Commentary)

Renatured (Comment) Organism
after denaturation by acid precipitation at pH 4.5 with HCl, recombining and neutralizing of precipitated protein and yellow supernatant results in a reconstitution of over 90% of the original activity Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.83
-
-
Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nicotinate + H2O + electron acceptor flavin may be the primary electron acceptor, tetrazolium dye as electron acceptor Bacillus sp. (in: Bacteria) 6-hydroxynicotinate + reduced electron acceptor
-
?
nicotinate + H2O + electron acceptor first step of nicotinate catabolism Bacillus sp. (in: Bacteria) 6-hydroxynicotinate + reduced electron acceptor
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Bacillus sp. (in: Bacteria)

Cofactor

Cofactor Comment Organism Structure
flavin primary electron acceptor, 1.5-2 mol tightly bound flavin per mol of enzyme Bacillus sp. (in: Bacteria)
additional information approximately 8 molecules tightly bound iron per enzyme molecule Bacillus sp. (in: Bacteria)