Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 1 mM, 30-50% inhibition | Bacillus sp. (in: Bacteria) | |
2,2'-dipyridyl | 0.1 mM, 30-50% inhibition | Bacillus sp. (in: Bacteria) | |
Atabrine | 1 mM, 30-50% inhibition | Bacillus sp. (in: Bacteria) | |
NaCl | sufficient NaCl inhibits enzyme activity significantly | Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
nicotinate | - |
Bacillus sp. (in: Bacteria) |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Bacillus sp. (in: Bacteria) | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
400000 | - |
gel filtration | Bacillus sp. (in: Bacteria) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotinate + H2O + electron acceptor | Bacillus sp. (in: Bacteria) | first step of nicotinate catabolism | 6-hydroxynicotinate + reduced electron acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
- |
- |
Purification (Comment) | Organism |
---|---|
24.4fold purification | Bacillus sp. (in: Bacteria) |
Renatured (Comment) | Organism |
---|---|
after denaturation by acid precipitation at pH 4.5 with HCl, recombining and neutralizing of precipitated protein and yellow supernatant results in a reconstitution of over 90% of the original activity | Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.83 | - |
- |
Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotinate + H2O + electron acceptor | flavin may be the primary electron acceptor, tetrazolium dye as electron acceptor | Bacillus sp. (in: Bacteria) | 6-hydroxynicotinate + reduced electron acceptor | - |
? | |
nicotinate + H2O + electron acceptor | first step of nicotinate catabolism | Bacillus sp. (in: Bacteria) | 6-hydroxynicotinate + reduced electron acceptor | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Bacillus sp. (in: Bacteria) |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | primary electron acceptor, 1.5-2 mol tightly bound flavin per mol of enzyme | Bacillus sp. (in: Bacteria) | |
additional information | approximately 8 molecules tightly bound iron per enzyme molecule | Bacillus sp. (in: Bacteria) |