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Literature summary for 1.17.1.5 extracted from
- Cloning, expression and functional analysis of nicotinate dehydrogenase gene cluster from Comamonas testosteroni JA1 that can hydroxylate 3-cyanopyridine (2010), Biodegradation, 21, 593-602.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ndhL, belongs to the ndh cluster with genes ndhS and ndhM, DNA and amino acid sequence determination, sequence comparison, recombinant expression of the nicotinate dehydrogenase gene cluster as His-tagged proteins in Pseudomonas entomophila strain L48, subcloning in Escherichia coli strain DH10B | Comamonas testosteroni |
| gene ndhM, belongs to the ndh cluster with genes ndhL and ndhS, DNA and amino acid sequence determination, sequence comparison, recombinant expression of the nicotinate dehydrogenase gene cluster as His-tagged proteins in Pseudomonas entomophila strain L48, subcloning in Escherichia coli strain DH10B | Comamonas testosteroni |
| gene ndhS, belongs to the ndh cluster with genes ndhL and ndhM, DNA and amino acid sequence determination, sequence comparison, recombinant expression of the nicotinate dehydrogenase gene cluster as His-tagged proteins in Pseudomonas entomophila strain L48, subcloning in Escherichia coli strain DH10B | Comamonas testosteroni |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | when the small unit of NaDHJA1 is replaced by the small subunit from NaDHKT2440 of Pseudomonas putida KT2440, the hybrid protein is able to catalyze the hydroxylation of nicotinate, but loses the ability to catalyze hydroxylation of 3-cyanopyridine | Comamonas testosteroni |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | the enzyme contains a [2Fe2S] domain, a type II [2Fe-2S] motif and a type I [2Fe-2S] motif, overview | Comamonas testosteroni |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20981 | - |
x * 20981, NdhS, sequence calculation | Comamonas testosteroni |
| 46042 | - |
x * 46042, NdhM, sequence calculation | Comamonas testosteroni |
| 82389 | - |
x * 82389, NdhL, sequence calculation | Comamonas testosteroni |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-cyanopyridine + H2O + NADP+ | Comamonas testosteroni | - |
6-hydroxy-3-cyanopyridine + NADPH + H+ | - |
? | |
| 3-cyanopyridine + H2O + NADP+ | Comamonas testosteroni JA1 | - |
6-hydroxy-3-cyanopyridine + NADPH + H+ | - |
? | |
| nicotinate + H2O + NADP+ | Comamonas testosteroni | - |
6-hydroxynicotinate + NADPH + H+ | - |
? | |
| nicotinate + H2O + NADP+ | Comamonas testosteroni JA1 | - |
6-hydroxynicotinate + NADPH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Comamonas testosteroni | B1PHD1 | ndhS; gene ndhS, operon ndhSLM | - |
| Comamonas testosteroni | B1PHD2 | ndhL; gene ndhL, operon ndhSLM | - |
| Comamonas testosteroni | B1PHD3 | ndhM; gene ndhM, operon ndhSLM | - |
| Comamonas testosteroni JA1 | B1PHD1 | ndhS; gene ndhS, operon ndhSLM | - |
| Comamonas testosteroni JA1 | B1PHD2 | ndhL; gene ndhL, operon ndhSLM | - |
| Comamonas testosteroni JA1 | B1PHD3 | ndhM; gene ndhM, operon ndhSLM | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-cyanopyridine + H2O + NADP+ | - |
Comamonas testosteroni | 6-hydroxy-3-cyanopyridine + NADPH + H+ | - |
? | |
| 3-cyanopyridine + H2O + NADP+ | - |
Comamonas testosteroni JA1 | 6-hydroxy-3-cyanopyridine + NADPH + H+ | - |
? | |
| nicotinate + H2O + NADP+ | - |
Comamonas testosteroni | 6-hydroxynicotinate + NADPH + H+ | - |
? | |
| nicotinate + H2O + NADP+ | - |
Comamonas testosteroni JA1 | 6-hydroxynicotinate + NADPH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 20981, NdhS, sequence calculation | Comamonas testosteroni |
| ? | x * 46042, NdhM, sequence calculation | Comamonas testosteroni |
| ? | x * 82389, NdhL, sequence calculation | Comamonas testosteroni |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NdhL | - |
Comamonas testosteroni |
| NdhM | - |
Comamonas testosteroni |
| NdhS | - |
Comamonas testosteroni |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Comamonas testosteroni |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Comamonas testosteroni |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Comamonas testosteroni | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | when the small unit of NaDHJA1 is replaced by the small subunit from NaDHKT2440 of Pseudomonas putida KT2440, the hybrid protein is able to catalyze the hydroxylation of nicotinate, but loses the ability to catalyze hydroxylation of 3-cyanopyridine | Comamonas testosteroni |
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