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Literature summary for 1.17.1.8 extracted from

  • Reddy, S.G.; Sacchettini, J.C.; Blanchard, J.S.
    Expression, purification, and characterization of Escherichia coli dihydrodipicolinate reductase (1995), Biochemistry, 34, 3492-3501.
    View publication on PubMed

Application

Application Comment Organism
medicine design and evaluation of dihydropicolinate reductase inhibitors as broad spectrum antibiotics and herbicides Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
dapB gene, overexpression from plasmid in Escherichia coli Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
2,6-pyridinedicarboxylic acid dead-end inhibitor Escherichia coli
NADP+ product inhibition Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0016
-
NADH
-
Escherichia coli
0.0021
-
thio-NADPH
-
Escherichia coli
0.004
-
reduced 3-acetylpyridine adenine dinucleotide
-
Escherichia coli
0.0056
-
NADPH
-
Escherichia coli
0.009
-
reduced nicotinamide hypoxanthine dinucleotide phosphate
-
Escherichia coli
0.05
-
2,3-dihydrodipicolinate
-
Escherichia coli
0.052
-
alpha-NADPH
-
Escherichia coli
0.08
-
3'-NADPH
-
Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
12000
-
gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,3-dihydrodipicolinate + NAD(P)H Escherichia coli lysine and diaminopimelate biosynthesis pathway 2,3,4,5-tetrahydrodipicolinate + NAD(P)+
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
overexpression in Escherichia coli from plasmid Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)+ + H2O = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H + H+ stereospecific transfer of the 4R hydrogen atom of the reduced nucleotide, as a hydrid ion, to the 4 position of dihydrodipicolinate Escherichia coli
(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)+ + H2O = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H + H+ kinetic and chemical mechanism Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
398
-
purified enzyme Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-dihydrodipicolinate + 3'-NADPH + H+
-
Escherichia coli 2,3,4,5-tetrahydrodipicolinate + 3'-NADP+ + H2O
-
r
2,3-dihydrodipicolinate + alpha-NADPH + H+
-
Escherichia coli 2,3,4,5-tetrahydrodipicolinate + alpha-NADP+ + H2O
-
r
2,3-dihydrodipicolinate + NAD(P)H
-
Escherichia coli 2,3,4,5-tetrahydrodipicolinate + NAD(P)+
-
?
2,3-dihydrodipicolinate + NAD(P)H lysine and diaminopimelate biosynthesis pathway Escherichia coli 2,3,4,5-tetrahydrodipicolinate + NAD(P)+
-
?
2,3-dihydrodipicolinate + reduced 3-acetylpyridine adenine dinucleotide
-
Escherichia coli 2,3,4,5-tetrahydrodipicolinate + oxidized 3-acetylpyridine adenine dinucleotide
-
?
2,3-dihydrodipicolinate + reduced 3-acetylpyridine adenine dinucleotide
-
Escherichia coli 2,3,4,5-tetrahydrodipicolinate + oxidized 3-acetylpyridine adenine dinucleotide + H2O
-
r
2,3-dihydrodipicolinate + reduced nicotinamide hypoxanthine dinucleotide phosphate
-
Escherichia coli 2,3,4,5-tetrahydrodipicolinate + oxidized nicotinamide hypoxanthine dinucleotide phosphate + H2O
-
r
2,3-dihydrodipicolinate + thio-NADPH + H+
-
Escherichia coli 2,3,4,5-tetrahydrodipicolinate + thio-NADP+ + H2O
-
r

Subunits

Subunits Comment Organism
tetramer 4 * 28000-28758, SDS-PAGE and electrospray mass spectrometry Escherichia coli

Synonyms

Synonyms Comment Organism
DHPR
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
additional information stereospecific transfer of the 4R hydrogen atom of the reduced nucleotide, as a hydrid ion, to the 4 position of dihydrodipicolinate Escherichia coli
NADH twice as effective as NADPH Escherichia coli
NADPH
-
Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.33
-
2,6-pyridinedicarboxylic acid
-
Escherichia coli
24
-
NADP+
-
Escherichia coli