Cloned (Comment) | Organism |
---|---|
gene dapB, expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
purified recombinant wild-type enzyme in ternary complex with inhibitor 2,6-pyridinedicarboxylate and NADH or NADPH, hanging drop vapour diffusion method, room temperature, 0.003 ml protein solution containing 20 mg/ml enzyme, 2 mM NADH or NADPH, and 30 mM 2,6-pyridinedicarboxylate, mixed with 0.003 ml precipitant solution containing 2.2 M ammonium sulfate, 0.1 M HEPES, pH 7.5, 3% PEG 400, 3 days, X-ray diffraction strcuture determination at 16°C and 2.2 A resolution and analysis | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
K11A | site-directed mutagenesis, increased activity with and specificty for NADH as cofactor, reduced activity with NADPH compared to the wild-type enzyme | Mycobacterium tuberculosis |
K9A | site-directed mutagenesis, increased specificty for NADH as cofactor, reduced activity with NADPH compared to the wild-type enzyme | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,6-pyridinedicarboxylate | competitive, binding site structure | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
NADH | recombinant mutant K9A | Mycobacterium tuberculosis | |
0.0032 | - |
NADH | recombinant wild-type enzyme | Mycobacterium tuberculosis | |
0.0043 | - |
NADH | recombinant mutant K11A | Mycobacterium tuberculosis | |
0.0118 | - |
NADPH | recombinant wild-type enzyme | Mycobacterium tuberculosis | |
0.042 | - |
NADPH | recombinant mutant K9A | Mycobacterium tuberculosis | |
0.83 | - |
NADPH | recombinant mutant K11A | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-dihydrodipicolinate + NAD(P)H | Mycobacterium tuberculosis | second step in the biosynthesis of meso-diaminopimelate, a bacterial cell wall component, and is involved in L-lysine biosynthesis | 2,3,4,5-tetrahydrodipicolinate + NAD(P)+ | - |
? | |
2,3-dihydrodipicolinate + NAD(P)H | Mycobacterium tuberculosis H37Rv | second step in the biosynthesis of meso-diaminopimelate, a bacterial cell wall component, and is involved in L-lysine biosynthesis | 2,3,4,5-tetrahydrodipicolinate + NAD(P)+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WP23 | gene dapB | - |
Mycobacterium tuberculosis H37Rv | P9WP23 | gene dapB | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)+ + H2O = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H + H+ | substrate binding site structure | Mycobacterium tuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity of recombinant wild-type and mutant enzymes with NADH or NADPH as cofactors | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-dihydrodipicolinate + NAD(P)H | - |
Mycobacterium tuberculosis | 2,3,4,5-tetrahydrodipicolinate + NAD(P)+ | - |
? | |
2,3-dihydrodipicolinate + NAD(P)H | second step in the biosynthesis of meso-diaminopimelate, a bacterial cell wall component, and is involved in L-lysine biosynthesis | Mycobacterium tuberculosis | 2,3,4,5-tetrahydrodipicolinate + NAD(P)+ | - |
? | |
2,3-dihydrodipicolinate + NAD(P)H | - |
Mycobacterium tuberculosis H37Rv | 2,3,4,5-tetrahydrodipicolinate + NAD(P)+ | - |
? | |
2,3-dihydrodipicolinate + NAD(P)H | second step in the biosynthesis of meso-diaminopimelate, a bacterial cell wall component, and is involved in L-lysine biosynthesis | Mycobacterium tuberculosis H37Rv | 2,3,4,5-tetrahydrodipicolinate + NAD(P)+ | - |
? | |
2,3-dihydrodipicolinate + NADH + H+ | - |
Mycobacterium tuberculosis | 2,3,4,5-tetrahydrodipicolinate + NAD+ + H2O | - |
r | |
2,3-dihydrodipicolinate + NADH + H+ | - |
Mycobacterium tuberculosis H37Rv | 2,3,4,5-tetrahydrodipicolinate + NAD+ + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | homotetramer, crystal structure | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
DHPR | - |
Mycobacterium tuberculosis |
dihydrodipicolinate reductase | - |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | dependent on | Mycobacterium tuberculosis | |
NADPH | dependent on | Mycobacterium tuberculosis |