Literature summary for 1.17.1.8 extracted from

Dommaraju, S.R.; Dogovski, C.; Czabotar, P.E.; Hor, L.; Smith, B.J.; Perugini, M.A.
Catalytic mechanism and cofactor preference of dihydrodipicolinate reductase from methicillin-resistant Staphylococcus aureus (2011), Arch. Biochem. Biophys., 512, 167-174.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Staphylococcus aureus

Inhibitors

Inhibitors	Comment	Organism	Structure
dihydrodipicolinate	the enzyme is inhibited by high concentrations of dihydrodipicolinate when using NADPH as a cofactor, but not with NADH	Staphylococcus aureus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.012	-	NADPH	in HEPES buffer, pH 8.0, at 30°C	Staphylococcus aureus
0.026	-	NADH	in HEPES buffer, pH 8.0, at 30°C	Staphylococcus aureus
0.028	-	(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate	using NADPH as cosubstrate, in HEPES buffer, pH 8.0, at 30°C	Staphylococcus aureus
0.039	-	(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate	using NADH as cosubstrate, in HEPES buffer, pH 8.0, at 30°C	Staphylococcus aureus

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	-	-	-
Staphylococcus aureus MRSA	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADH + H+	-	Staphylococcus aureus	(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD+ + H2O	-	ir
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADH + H+	-	Staphylococcus aureus MRSA	(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD+ + H2O	-	ir
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADPH + H+	-	Staphylococcus aureus	(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NADP+ + H2O	-	ir
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADPH + H+	-	Staphylococcus aureus MRSA	(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NADP+ + H2O	-	ir

Synonyms

Synonyms	Comment	Organism
DHDPR	-	Staphylococcus aureus
dihydrodipicolinate reductase	-	Staphylococcus aureus
EC 1.3.1.26	formerly	Staphylococcus aureus

Cofactor

Cofactor	Comment	Organism	Structure
NADH	-	Staphylococcus aureus
NADPH	NADPH is a more efficient cofactor than NADH	Staphylococcus aureus

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Release 2023.1 (January 2023)