Literature summary for 1.17.1.8 extracted from

Girish, T.S.; Navratna, V.; Gopal, B.
Structure and nucleotide specificity of Staphylococcus aureus dihydrodipicolinate reductase (DapB) (2011), FEBS Lett., 585, 2561-2567.

Search for more literature for 1.17.1.8

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Staphylococcus aureus

Crystallization (Commentary)

Crystallization (Comment)	Organism
using 2.0 M ammonium sulfate and 0.2 M sodium acetate pH 4.5	Staphylococcus aureus

Protein Variants

Protein Variants	Comment	Organism
K35A	the mutation does not alter the oligomeric status or spectroscopic properties of the enzyme, however, NADPH binding is altered by ca 20fold with much less change (ca 4fold) in NADH binding compared to the wild type enzyme	Staphylococcus aureus

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	Q5HG24	-	-
Staphylococcus aureus COL	Q5HG24	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+-NTA affinity column chromatography and DEAE-Sepharose column chromatography	Staphylococcus aureus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADH + H+	-	Staphylococcus aureus	(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD+ + H2O	-	ir
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADH + H+	-	Staphylococcus aureus COL	(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD+ + H2O	-	ir
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADPH + H+	-	Staphylococcus aureus	(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NADP+ + H2O	-	ir
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADPH + H+	-	Staphylococcus aureus COL	(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NADP+ + H2O	-	ir

Synonyms

Synonyms	Comment	Organism
DapB	-	Staphylococcus aureus
DHDPR	-	Staphylococcus aureus
dihydrodipicolinate reductase	-	Staphylococcus aureus

Cofactor

Cofactor	Comment	Organism	Structure
NADH	-	Staphylococcus aureus
NADPH	NADPH is a more preferred co-factor than NADH	Staphylococcus aureus

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Release 2023.1 (January 2023)