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Literature summary for 1.17.2.2 extracted from

  • Hopper, D.J.; Rogozinski, J.; Toczko, M.
    Lupanine hydroxylase, a quinocytochrome c from an alkaloid-degrading Pseudomonas sp. (1991), Biochem. J., 279, 105-109.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.17.2.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6
-
 lupanine 25°C, pH 8.5 Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
 ultracentrifugation studies Pseudomonas sp.
72000
-
 1 * 72000, SDS-PAGE Pseudomonas sp.
74000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lupanine + pyrroloquinoline quinone + H2O Pseudomonas sp. lupanine is a quinolizidine alkaloid and is particularly abundant in species of the genus Lupinus. It can be degraded by bacteria. The first step in its catabolism by a Pseudomonas sp. is hydroxylation to 17-hydroxylupanine 17-hydroxylupanine + pyrroloquinoline quinol
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
69.7
-
 25°C, pH 8.5 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lupanine + pyrroloquinoline quinone + H2O
-
 Pseudomonas sp. 17-hydroxylupanine + pyrroloquinoline quinol
-
 ?
lupanine + pyrroloquinoline quinone + H2O lupanine is a quinolizidine alkaloid and is particularly abundant in species of the genus Lupinus. It can be degraded by bacteria. The first step in its catabolism by a Pseudomonas sp. is hydroxylation to 17-hydroxylupanine Pseudomonas sp. 17-hydroxylupanine + pyrroloquinoline quinol
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 72000, SDS-PAGE Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
21.3
-
 lupanine 25°C, pH 8.5 Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
7 10 enzyme is assayed over a range of pH from 7 to 10 in sodium/potassium phosphate, Tris/HCI and glycine/NaOH buffers Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
heme contains one heme group per molecule of enzyme Pseudomonas sp.
pyrroloquinoline quinone contains one molecule of pyrroloquinoline quinone per molecule of enzyme Pseudomonas sp.

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas sp. isoelectric focusing, pH 3-10, reduced enzyme
-
 5
Pseudomonas sp. isoelectric focusing, pH 3-10, oxidized enzyme
-
 5.3