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Literature summary for 1.17.3.2 extracted from

  • Woolfolk, C.A.; Downard, J.S.
    Bacterial xanthine oxidase from Arthrobacter S-2 (1978), J. Bacteriol., 135, 422-428.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.17.3.2

General Stability

General Stability Organism
stable for more than 1 year with alternate freezing and thawing Arthrobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
xanthine no substrate inhibition Arthrobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Arthrobacter sp.
0.13
-
 xanthine
-
 Arthrobacter sp.
0.13
-
 1-Methylxanthine
-
 Arthrobacter sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Iron iron-molybdenum protein Arthrobacter sp.
Molybdenum an iron-molybdenum protein Arthrobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 2 * 80000, SDS-PAGE Arthrobacter sp.
146000
-
 gel filtration Arthrobacter sp.

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
 S-2
-
Arthrobacter sp. S-2
-
 S-2
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information
-
 Arthrobacter sp.
proteolytic modification
-
 Arthrobacter sp.

Purification (Commentary)

Purification (Comment) Organism
-
 Arthrobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
230
-
-
 Arthrobacter sp.

Storage Stability

Storage Stability Organism
-75°C, more than 1 year with alternate freezing and thawing, stable Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-methylxanthine + H2O + O2
-
 Arthrobacter sp. 1-methylurate + H2O2
-
 ?
hypoxanthine + 2 H2O + 2 O2
-
 Arthrobacter sp. urate + 2 H2O2
-
 ?
hypoxanthine + 2 H2O + 2 O2
-
 Arthrobacter sp. S-2 urate + 2 H2O2
-
 ?
xanthine + H2O + O2 electron acceptor O2 Arthrobacter sp. uric acid + H2O2
-
 ?
xanthine + H2O + O2 Arthrobacter S-2 enzyme: relatively specific Arthrobacter sp. uric acid + H2O2
-
 ?
xanthine + H2O + O2 electron acceptor ferricyanide Arthrobacter sp. uric acid + H2O2
-
 ?
xanthine + H2O + O2 electron acceptor 2,6-dichlorophenolindophenol Arthrobacter sp. uric acid + H2O2
-
 ?
xanthine + H2O + O2 electron acceptor O2 Arthrobacter sp. S-2 uric acid + H2O2
-
 ?
xanthine + H2O + O2 Arthrobacter S-2 enzyme: relatively specific Arthrobacter sp. S-2 uric acid + H2O2
-
 ?
xanthine + H2O + O2 electron acceptor ferricyanide Arthrobacter sp. S-2 uric acid + H2O2
-
 ?
xanthine + H2O + O2 electron acceptor 2,6-dichlorophenolindophenol Arthrobacter sp. S-2 uric acid + H2O2
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 80000, SDS-PAGE Arthrobacter sp.

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein Arthrobacter sp.