Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-coumaric acid | competitively inhibits the enzyme by binding to the active site, have a protective effect against reactive oxygen species in cells, structure-function relationship, computational molecular docking, overview | Mus musculus | |
2-methoxycinnamic acid | competitively inhibits the enzyme by binding to the active site, have a protective effect against reactive oxygen species in cells, structure-function relationship, computational molecular docking, overview | Mus musculus | |
3-coumaric acid | competitively inhibits the enzyme by binding to the active site, have a protective effect against reactive oxygen species in cells, structure-function relationship, computational molecular docking, overview | Mus musculus | |
3-methoxycinnamic acid | competitively inhibits the enzyme by binding to the active site, have a protective effect against reactive oxygen species in cells, structure-function relationship, computational molecular docking, overview | Mus musculus | |
4-coumaric acid | competitively inhibits the enzyme by binding to the active site, have a protective effect against reactive oxygen species in cells, structure-function relationship, computational molecular docking, overview | Mus musculus | |
4-methoxycinnamic acid | competitively inhibits the enzyme by binding to the active site, have a protective effect against reactive oxygen species in cells, structure-function relationship, computational molecular docking, overview | Mus musculus | |
allopurinol | 36.6% inhibition at 0.04 mM | Mus musculus | |
aminoguanidine | 78.6% inhibition at 0.04 mM | Mus musculus | |
ascorbate | 43.5% inhibition at 0.04 mM | Mus musculus | |
caffeic acid | 42.8% inhibition at 0.04 mM, competitively inhibits the enzyme by binding to the active site, have a protective effect against reactive oxygen species in cells, structure-function relationship, computational molecular docking, overview | Mus musculus | |
caffeic acid phenethyl ester | 55.0% inhibition at 0.004 mM, competitively inhibits the enzyme by binding to the active site, have a protective effect against reactive oxygen species in cells, structure-function relationship, computational molecular docking, overview | Mus musculus | |
Cinnamic acid | 40.2% inhibition at 0.04 mM, competitively inhibits the enzyme by binding to the active site, have a protective effect against reactive oxygen species in cells, structure-function relationship, computational molecular docking, overview | Mus musculus | |
ferulic acid | competitively inhibits the enzyme by binding to the active site, have a protective effect against reactive oxygen species in cells, structure-function relationship, computational molecular docking, overview | Mus musculus | |
isoferulic acid | competitively inhibits the enzyme by binding to the active site, have a protective effect against reactive oxygen species in cells, structure-function relationship, computational molecular docking, overview | Mus musculus | |
additional information | inhibitory effects of phenylpropanoids on DNA relaxation activities, 1,1-diphenyl-2-picrylhydrazyl, and 5,5-dimethyl-1-pyrroline-N-oxide, and on iron-induced hydroxyl radical formation, hydroxyl radical-scavenger properties of the compounds, overview, 3D modelling of docking | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
xanthine + H2O + O2 | Mus musculus | production of superoxide radicals, treatment with an enzyme inhibitor largely prevents the development of endothelial dysfunction and atherosclerosis in mice | urate + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
RAW-264.7 cell | a mouse monocyte-macrophage cell line, ATCC TIB-71 | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
xanthine + H2O + O2 | production of superoxide radicals | Mus musculus | urate + H2O2 | - |
? | |
xanthine + H2O + O2 | production of superoxide radicals, treatment with an enzyme inhibitor largely prevents the development of endothelial dysfunction and atherosclerosis in mice | Mus musculus | urate + H2O2 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Mus musculus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00143 | - |
pH 8.0, 37°C, competitive versus xanthine | Mus musculus | allopurinol | |
0.00626 | - |
pH 8.0, 37°C, competitive versus xanthine | Mus musculus | caffeic acid phenethyl ester | |
0.0654 | - |
pH 8.0, 37°C, competitive versus xanthine | Mus musculus | caffeic acid | |
0.0654 | - |
pH 8.0, 37°C, versus xanthine | Mus musculus | caffeic acid | |
0.0939 | - |
pH 8.0, 37°C, versus xanthine | Mus musculus | ferulic acid | |
0.0969 | - |
pH 8.0, 37°C, versus xanthine | Mus musculus | 4-coumaric acid | |
0.1432 | - |
pH 8.0, 37°C, versus xanthine | Mus musculus | isoferulic acid | |
0.1567 | - |
pH 8.0, 37°C, inhibition of hydroxylradical formation in the dimethyl-1-pyrroline-N-oxide spin-trapping assay | Mus musculus | caffeic acid phenethyl ester | |
0.184 | - |
pH 8.0, 37°C, versus xanthine | Mus musculus | 4-methoxycinnamic acid |