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Literature summary for 1.17.3.2 extracted from

  • Jankowska, D.A.; Trautwein-Schult, A.; Cordes, A.; Hoferichter, P.; Klein, C.; Bode, R.; Baronian, K.; Kunze, G.
    Arxula adeninivorans xanthine oxidoreductase and its application in the production of food with low purine content (2013), J. Appl. Microbiol., 115, 796-807.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0508
-
hypoxanthine pH 8.5, 43°C Blastobotrys adeninivorans
0.0728
-
xanthine pH 8.5, 43°C Blastobotrys adeninivorans
0.162
-
NAD+ pH 8.5, 43°C Blastobotrys adeninivorans
0.208
-
purine pH 8.5, 43°C Blastobotrys adeninivorans

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ inhibitory effect Blastobotrys adeninivorans
Cu2+ strong inhibition Blastobotrys adeninivorans
Fe2+ inhibitory effect Blastobotrys adeninivorans
Zn2+ inhibitory effect Blastobotrys adeninivorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000
-
SDS-PAGE Blastobotrys adeninivorans
360000
-
gel filtration Blastobotrys adeninivorans

Organism

Organism UniProt Comment Textmining
Blastobotrys adeninivorans R4ZGN4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hypoxanthine + NAD+ + H2O
-
Blastobotrys adeninivorans xanthine + NADH + H+
-
?
pterine + NAD+
-
Blastobotrys adeninivorans ? + H2O + NADH
-
?
purine + NAD+
-
Blastobotrys adeninivorans ? + H2O + NADH
-
?
xanthine + H2O + O2
-
Blastobotrys adeninivorans urate + H2O2
-
?
xanthine + NAD+ + H2O
-
Blastobotrys adeninivorans urate + NADH
-
?

Synonyms

Synonyms Comment Organism
AXOR
-
Blastobotrys adeninivorans
xanthine oxidoreductase
-
Blastobotrys adeninivorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
43
-
-
Blastobotrys adeninivorans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 50
-
Blastobotrys adeninivorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
5% activity in 1 h at 30°C Blastobotrys adeninivorans
40
-
12% activity in 1 h at 40°C Blastobotrys adeninivorans
50
-
22% activity in 1 h at 50°C Blastobotrys adeninivorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
glycine-NaOH buffer Blastobotrys adeninivorans

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9.5 potassium phosphate, Tris-HCl, Tris-acetate and glycine-NaOH buffer Blastobotrys adeninivorans

General Information

General Information Comment Organism
malfunction deletion mutant exhibits less than 1% of the wild-type strain activity towards xanthine and hypoxanthine under inducible conditions and both strains show no activity after cultivation without inducer (adenine) Blastobotrys adeninivorans