KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0508 | - |
hypoxanthine | pH 8.5, 43°C | Blastobotrys adeninivorans | |
0.0728 | - |
xanthine | pH 8.5, 43°C | Blastobotrys adeninivorans | |
0.162 | - |
NAD+ | pH 8.5, 43°C | Blastobotrys adeninivorans | |
0.208 | - |
purine | pH 8.5, 43°C | Blastobotrys adeninivorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | inhibitory effect | Blastobotrys adeninivorans | |
Cu2+ | strong inhibition | Blastobotrys adeninivorans | |
Fe2+ | inhibitory effect | Blastobotrys adeninivorans | |
Zn2+ | inhibitory effect | Blastobotrys adeninivorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
160000 | - |
SDS-PAGE | Blastobotrys adeninivorans |
360000 | - |
gel filtration | Blastobotrys adeninivorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Blastobotrys adeninivorans | R4ZGN4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hypoxanthine + NAD+ + H2O | - |
Blastobotrys adeninivorans | xanthine + NADH + H+ | - |
? | |
pterine + NAD+ | - |
Blastobotrys adeninivorans | ? + H2O + NADH | - |
? | |
purine + NAD+ | - |
Blastobotrys adeninivorans | ? + H2O + NADH | - |
? | |
xanthine + H2O + O2 | - |
Blastobotrys adeninivorans | urate + H2O2 | - |
? | |
xanthine + NAD+ + H2O | - |
Blastobotrys adeninivorans | urate + NADH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AXOR | - |
Blastobotrys adeninivorans |
xanthine oxidoreductase | - |
Blastobotrys adeninivorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
43 | - |
- |
Blastobotrys adeninivorans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 50 | - |
Blastobotrys adeninivorans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
5% activity in 1 h at 30°C | Blastobotrys adeninivorans |
40 | - |
12% activity in 1 h at 40°C | Blastobotrys adeninivorans |
50 | - |
22% activity in 1 h at 50°C | Blastobotrys adeninivorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
glycine-NaOH buffer | Blastobotrys adeninivorans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | potassium phosphate, Tris-HCl, Tris-acetate and glycine-NaOH buffer | Blastobotrys adeninivorans |
General Information | Comment | Organism |
---|---|---|
malfunction | deletion mutant exhibits less than 1% of the wild-type strain activity towards xanthine and hypoxanthine under inducible conditions and both strains show no activity after cultivation without inducer (adenine) | Blastobotrys adeninivorans |