Literature summary for 1.17.4.1 extracted from

Sinhababu, A.K.; Boehlert, C.C.; Gan, L.S.; Yanni, S.B.; Thakker, D.R.
High-performance liquid chromatographic purification, optimization of the assay, and properties of ribonucleoside diphosphate reductase from rabbit bone marrow (1995), Arch. Biochem. Biophys., 317, 285-291.

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Activating Compound

Activating Compound	Comment	Organism	Structure
2-mercaptoethanol	25 mM, maximal activation, 70% of activity with dithiothreitol	Oryctolagus cuniculus
dithioerythritol	0.5 mM, maximal activation, 94% of activity with dithiothreitol	Oryctolagus cuniculus
dithiothreitol	optimal in vitro activity with 10 mM, inhibition above	Oryctolagus cuniculus

Inhibitors

Inhibitors	Comment	Organism	Structure
dithiothreitol	higher than 10 mM, activation below	Oryctolagus cuniculus
Hydroxyurea	-	Oryctolagus cuniculus
UDP	inhibition of CDP reduction	Oryctolagus cuniculus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0014	-	CDP	in the presence of 5'-adenylimidodiphosphate	Oryctolagus cuniculus
0.0023	-	CDP	in the presence of ATP	Oryctolagus cuniculus

Organism

Organism	UniProt	Comment	Textmining
Oryctolagus cuniculus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
gel filtration, ATP-agarose, partial purification	Oryctolagus cuniculus

Source Tissue

Source Tissue	Comment	Organism	Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0000045	-	partially purified enzyme, CDP reduction	Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
CDP + reduced dithiothreitol	-	Oryctolagus cuniculus	2'-dCDP + oxidized dithiothreitol + H2O	-	r
CDP + reduced dithiothreitol	-	Oryctolagus cuniculus	dCDP + oxidized dithiothreitol + H2O	-	r
ribonucleoside diphosphate + reduced thioredoxin	-	Oryctolagus cuniculus	2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O	-	ir

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.029	-	UDP	-	Oryctolagus cuniculus
0.69	-	Hydroxyurea	-	Oryctolagus cuniculus

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Release 2023.1 (January 2023)