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Literature summary for 1.17.4.1 extracted from

  • Jiang, W.; Xie, J.; N?rgaard, H.; Bollinger, J.M.; Krebs, C.
    Rapid and quantitative activation of Chlamydia trachomatis ribonucleotide reductase by hydrogen peroxide (2008), Biochemistry, 47, 4477-4483.
    View publication on PubMed
Search for more literature for 1.17.4.1

Activating Compound

Activating Compound Comment Organism Structure
ATP
-
 Chlamydia trachomatis
DTT
-
 Chlamydia trachomatis
additional information quantitative activation Chlamydia trachomatis

Application

Application Comment Organism
pharmacology inhibition of RNRs is a proven strategy for combating cancer and some viruses Chlamydia trachomatis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics, overview Chlamydia trachomatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CDP + thioredoxin Chlamydia trachomatis
-
 2'-deoxyCDP + thioredoxin disulfide + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Chlamydia trachomatis
-
 a human pathogen
-

Oxidation Stability

Oxidation Stability Organism
the enzyme is not stable to to reactive oxygen and nitrogen species, RO(N)S, produced by the host’s immune system Chlamydia trachomatis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CDP + thioredoxin
-
 Chlamydia trachomatis 2'-deoxyCDP + thioredoxin disulfide + H2O
-
 ?

Subunits

Subunits Comment Organism
tetramer 2 * R1 subunit + 2 * R2 subunit Chlamydia trachomatis

Synonyms

Synonyms Comment Organism
More a class Ic ribonucleotide reductase Chlamydia trachomatis
RNR
-
 Chlamydia trachomatis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at Chlamydia trachomatis

Cofactor

Cofactor Comment Organism Structure
manganese-iron cofactor the class Ic RNR from Chlamydia trachomatis uses a Mn(IV)/Fe(III) cofactor, with high specificity for MnIV, which functionally replaces the tyrosyl radical used by conventional class I RNRs to initiate substrate radical production. The intermediate decays by reduction of the Fe site to the active MnIV/FeIII-R2 complex. The reaction of the MnII/FeII-R2 species with H2O2 proceeds in three resolved steps: sequential oxidation to MnIII/FeIII-R2 and Mn(IV)/Fe(IV)-R2, followed by decay of the intermediate to the active Mn(IV)/Fe(III)-R2 product, kinetics and reaction mechanism, overview Chlamydia trachomatis
thioredoxin
-
 Chlamydia trachomatis