Inhibitors | Comment | Organism | Structure |
---|---|---|---|
but-3-ynyl diphosphate | - |
Aquifex aeolicus | |
additional information | analysis of alkynyl diphosphate inhibitors, inhibition model based on an electrostatic interaction with the active site E126, model for alkyne inhibition involving pi (or pi/sigma) metallacycle complex formation with the unique 4th Fe in the Fe4S4 cluster, computational docking and quantum chemical calculations, overview | Aquifex aeolicus | |
propargyl diphosphate | - |
Aquifex aeolicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | IspH contains a [4Fe-4S] cluster | Aquifex aeolicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | - |
gene ispH or lytB | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H + H+ | HMBPP reductase catalyzes the 2H+/2e- reduction of (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate to form an approximately 5:1 mixture of isopentenyl diphosphate and dimethylallyl diphosphate, insights into IspH catalysis and inhibition, involving organometallic species, overview | Aquifex aeolicus | dimethylallyl diphosphate + NAD(P)+ + H2O | - |
r | |
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H + H+ | HMBPP reductase catalyzes the 2H+/2e- reduction of (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate to form an approximately 5:1 mixture of isopentenyl diphosphate and dimethylallyl diphosphate, insights into IspH catalysis and inhibition, involving organometallic species, overview | Aquifex aeolicus | isopentenyl diphosphate + NAD(P)+ + H2O | - |
r |
Synonyms | Comment | Organism |
---|---|---|
HMBPP reductase | - |
Aquifex aeolicus |
ispH | - |
Aquifex aeolicus |
LytB | - |
Aquifex aeolicus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00045 | - |
pH not specified in the publication, temperature not specified in the publication | Aquifex aeolicus | but-3-ynyl diphosphate |